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CD163+ macrophages monitor enhanced permeability at the blood–dorsal root ganglion barrier

ABSTRACT: CD163+ macrophages monitor enhanced permeability at the blood–dorsal root ganglion barrier

PROVIDER: PRJNA1031952 | ENA |

REPOSITORIES: ENA

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CD163+ macrophages monitor enhanced permeability at the blood–dorsal root ganglion barrier

Project description:In dorsal root ganglia (DRG), macrophages reside in close proximity to sensory neurons, and their functions have largely been explored in the context of pain, nerve injury and repair. In this study, however, we discovered that the majority of macrophages in DRGs are in direct contact with the vasculature where they constantly monitor the circulation, efficiently phagocytosing proteins and macromolecules from the blood. Characterization of the DRG endothelium revealed a specialized vascular network spanning the arteriovenous axis, which gradually transformed from a barrier type endothelium in arteries to a highly permeable endothelium in veins. Macrophage phagocytosis spatially aligned with peak endothelial permeability and we identified caveolar transcytosis as a mechanism regulating endothelial permeability. Profiling of the DRG immune landscape revealed two subsets of perivascular macrophages with distinct transcriptome, turnover and function. CD163 expressing macrophages self-maintained locally, specifically participated in vasculature monitoring, displayed distinct responses during peripheral inflammation and were conserved in mouse and Man. Our work provides a molecular explanation for the permeability of the blood-DRG barrier and identifies an unappreciated role of macrophages as integral components of the DRG-neurovascular unit. DOI: 10.1084/jem.20230675

2023-11-23 | GSE246168 | GEO

Ribotag analysis of proprioceptive dorsal root ganglion neurons in cell culture

Project description:We analyzed ribosome-associated RNA (raRNA) in dorsal root ganglion neurons in TrkC+ proprioceptive dorsal root ganglion neurons in cell culture.

2018-04-25 | GSE110374 | GEO

RNA-Seq of single cells from the mouse lumbar dorsal root ganglion

Project description:In order to establish a consensus catalog of dorsal rott ganglion cell types, we used comprehensive transcriptome analysis of single cells for unsupervised identification and molecular classification of sensory neurons independent of any a priori knowledge of sensory subtypes. RNA-Seq was performed on 799 dissociated single cells dissected from the mouse lumbar dorsal root ganglion distributed over a total of nine 96-well plates

2014-11-24 | E-GEOD-59739 | biostudies-arrayexpress

Neuronal cell lines as model dorsal root ganglion neurons: a transcriptomic comparison

Project description:Aim: Dorsal root ganglion neuron-derived immortal cell lines including ND7/23 and F-11 cells have been used extensively as in vitro model systems of native peripheral sensory neurons. However, while it is clear that some sensory neuron-specific receptors and ion channels are present in these cell lines, a systematic comparison of the molecular targets expressed by these cell lines with intact peripheral neurons is lacking. Methods: we examined the expression of RNA transcripts in the human neuroblastoma-derived cell line, SH-SY5Y, and two dorsal root ganglion hybridoma cell lines, F-11 and ND7/23, using Illumina next-generation sequencing. Results: The expression profile of these three cell lines did not resemble any specific dorsal root ganglion neuron subclass. The cell lines lacked many markers for nociceptive sensory neurons, such as the transient receptor potential V1 gene, but expressed markers for both myelinated and unmyelinated neurons. Conclusion: This paper provides insights into the receptor repertoire expressed in common dorsal root ganglion neuron-derived cell lines, and illustrates the limits and potentials of these cell lines as tools for neuropharmacological exploration.

2016-05-01 | GSE75811 | GEO

RNA-sequencing of lidocaine treatment on rat dorsal root ganglion neurons

Project description:We conducted RNA-sequencing of lidocaine hydrochloride in treating rat dorsal root ganglion neurons to detect lidocaine’s effect of transcriptome profiling changes compared with control.

2019-11-09 | GSE140162 | GEO

MicroRNA profiling in dorsal root ganglion after sciatic nerve injury

Project description:Changes in microRNA (miRNA) expression in the mouse L4 and L5 dorsal root ganglion following unilateral sciatic nerve transection. The timepoint of 7 days post-axotomy was chosen to capture miRNA expression profiles at a time when the injured neurons were beginning to regenerate. Two condition experiment, paired control DRG vs axotomised DRG following unilateral sciatic nerve transection. 3 biological replicates, one replicate per array. Dye swap in Replicate 2.

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LncRNA sequencing in dorsal root ganglion cultured with high sugar content

Project description:Primary isolated rat dorsal root ganglion nerve cells (DRGs) were cultured with high glucose in vitro and divided into normal culture group and high glucose culture group

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RNA-sequencing to idendify axon RNAs in dentate gyrus granule cells, dorsal root ganglion cells and retinal ganglion cells

Project description:To analyze axon transcriptome of dentate gyrus granule cells, dorsal root ganglion cells and retinal ganglion cells, we perfomed axon RNA sequencing.Neurons were cultured in microfluidic chambers and axons were isolated. Total RNA of axons was collected separately and subjected to library construction using M01440v2 NuGEN Trio RNA-seq kit. After sequencencing,we used TPM method to normalize RNA-seq reads.

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Project description:Spatial transcriptomics on human dorsal root ganglion (DRG)

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Human Dorsal Root Ganglion PolyA RNA Seq Dataset

Project description:Human Dorsal Root Ganglion PolyA RNA Seq Dataset

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