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ABSTRACT: Adaptations to Deep-Sea Hydrothermal Vent Environments of Hydrogenimonaceae

PROVIDER: PRJNA1031963 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26511451_1.fastq.gz	Fastqsanger.gz
	SRR26511451_2.fastq.gz	Fastqsanger.gz
	SRR26511452_1.fastq.gz	Fastqsanger.gz
	SRR26511452_2.fastq.gz	Fastqsanger.gz
	SRR26511453_1.fastq.gz	Fastqsanger.gz

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Project description:Adaptations to Deep-Sea Hydrothermal Vent Environments of Hydrogenimonaceae

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Dissolved organic carbon compounds in deep-sea hydrothermal vent fluids from the East Pacific Rise at 9°50′N

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Project description:Physiological and gene expression studies of deep-sea bacteria under pressure conditions similar to those experienced in their natural habitat are critical to understand growth kinetics and metabolic adaptations to in situ conditions. The Epslilonproteobacterium, Nautilia sp. strain PV1, was isolated from hydrothermal fluids released from an active deep-sea hydrothermal vent at 9°N on the East Pacific Rise. Using a high pressure/high temperature continuous culture system we established that strain PV-1 has the shortest generation time of all known piezophilic microorganisms and we investigated its protein expression pattern in response to different hydrostatic pressures. Proteomic analyses of strain PV-1 grown at 200 Bars and 5 Bars showed that pressure adaptation is not restricted only to stress response or homeoviscous adaptation, but that it is more diversified and protein specific, with a fine and variegated regulation of enzymes involved even in the same metabolic pathway. As previously reported, proteins synthesis, motility, transport and energy metabolism are all affected by pressure, although to different extents. In strain PV-1, low pressure condition seems to activate the synthesis of phage-related proteins and an overexpression of enzymes involved in central carbon metabolism.

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