Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-sequencing of Mtb H37Rv untreated and H37Rv treated with 2XMIC CJ067

ABSTRACT: RNA-sequencing of Mtb H37Rv untreated and H37Rv treated with 2XMIC CJ067

PROVIDER: PRJNA1032589 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

RNA-sequencing of Mtb H37Rv untreated and H37Rv treated with 2XMIC CJ067

Project description:The ability ofMycobacterium tuberculosis(Mtb) to tolerate nitric oxide (NO) and superoxide (O2•−) produced by phagocytes contributes to its success as a human pathogen. Recombination of NO and O2•− generates peroxynitrite (ONOO-), a potent oxidant produced inside immunologically-activated macrophages and causes lethality in diverse organisms. However, while the response ofMtbtowards NO and O2•− is well established, howMtbresponds to ONOO- remains uncertain. Filling this knowledge gap is important to understand the persistence mechanisms of Mtb during infection Hence, we used an endogenous peroxynitrite donor CJ067 and treated Mtb to identify new mechanisms of ONOO- resistance in mycobacteria.

2024-09-13 | GSE246347 | GEO

Transcriptomic analysis of Mtb H37Rv on treatment with ATD-3169 (Redox generating compound)

Project description:Mtb H37Rv was found to be highly susceptible to ATD-3169 (Redox generating compound). To further understand the mechanism of ATD-3169 action, we performed microarray analysis of Mtb H37Rv exposed to 3µM, 30 µM and 150µM of ATD-3169 for 4h.

2014-11-30 | GSE61618 | GEO

miRNA profiles of Mtb H37Rv infected peritoneal macrophages and uninfected controls

Project description:The peritoneal macrophages were infected with Mtb H37Rv for 4 hours, and the miRNA expression profile were analyzed with deep sequencing.

2018-09-06 | GSE119495 | GEO

Temporal response of Mycobacterium tuberculosis H37Rv to vitamin C exposure in THP-1 macrophages.

Project description:Transcriptional profiling of Mtb H37Rv infected into THP-1 macrophage cell line and treated with 100 µM vitamin C (vit C) for 96 hours and 144 hours, compared to gene expression profile of untreated bacteria post-infection.

2021-10-01 | GSE179376 | GEO

RNA-sequencing of Mtb H37Rv, MtbΔiscS, iscS complemented, Mtb-sufS knockdown, ΔiscS-sufS knockdown

Project description:RNA-sequencing of Mtb H37Rv, MtbΔiscS, iscS complemented, Mtb-sufS knockdown, ΔiscS-sufS knockdown

| PRJNA929478 | ENA

miRNA profiles of lung tissues from Mtb H37Rv infected C57B/6 mice and uninfected controls

Project description:The C57B/6 mice were infected with Mtb H37Rv (CFU=200) for 28 days, and the miRNA expression profile from lung tissues were analyzed with deep sequencing.

2018-09-06 | GSE119496 | GEO

THP1 cell response to Mycobacterium tuberculosis infection and Mtb PAMP stimulation

Project description:To compare gene expression changes induced by infection with Mycobacterium tuberculosis (Mtb) with changes induced by purified Mtb products, we infected THP-1 cells with Mtb strain H37Rv or treated with purified Mtb products, then performed RNAseq.

2023-05-15 | GSE227851 | GEO

Transcriptomic analysis of untreated log-phase Mtb H37Rv, Mtbrv0158 Knockout strain and Mtbrv0158 complemented strain

Project description:Transcriptomic analysis of untreated log-phase Mtb H37Rv, Mtbrv0158 Knockout strain and Mtbrv0158 complemented strain

| PRJNA807531 | ENA

RNA-sequencing of Mtb H37Rv, MtbΔiscS, iscS complemented, Mtb-sufS knockdown, ΔiscS-sufS knockdown

Project description:Iron-sulfur (Fe-S) cluster containing proteins are a subset of proteins with crucial functions in the maintenance of cellular physiology throughout all kingdoms of life. The systems involved in the biogenesis and repair of Fe-S clusters hence plays important role in fine-tuning the availability and functionality of Fe-S proteins. Two of the systems known in bacteria are, Isc and Suf. Compared to the facultative anaerobe, E. coli, which codes for the two multi-genic Fe-S biogenesis systems; Mtb Fe-S biogenesis machinery is skewed with a multi-genic Suf system (sufRBDCSUT) and a single gene of Isc system (iscS). Several Fe-S proteins are deployed by Mtb to maintain cellular homeostasis and survival in a hostile host environment. Hence, we determine the transcriptome of Mtb on depletion of the two key enzymes of Fe-S biogenesis- IscS and sufS, that could help understand the role and regulation between the two systems in the human pathogen Mtb.

2023-10-01 | GSE224043 | GEO

Time-dependent global gene expression profile of RAW264.7 cells infected with Mycobacterium tuberculosis H37Rv (Mtb)

Project description:Transcriptional profiling of RAW264.7 cells infected with M. tuberculosis H37Rv at an MOI of 10 performed 4 and 24 hours post-infection. RAW264.7 cells were infected with Mtb for 4 hours at an MOI of 10. Cells were washed and treated with gentamycin for 2 hours in order to remove adhered bacteria. Incubation was continued further for 4 and 24 hours followed by total RNA isolation. Total RNA was labelled with Agilent’s quick-Amp labelling kit (p/n: 5190-0444) to generate fluorescent complementary RNA by using T7 promoter based linear amplification. The control sample was labelled with Cy3 while the infected samples were labelled with Cy5 and hybridized to an Agilent oligo microarray kit.

2014-12-23 | E-GEOD-64427 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data