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RNA-sequencing of Mtb H37Rv untreated and H37Rv treated with 2XMIC CJ067

ABSTRACT: RNA-sequencing of Mtb H37Rv untreated and H37Rv treated with 2XMIC CJ067

PROVIDER: PRJNA1032589 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26538069.fastq.gz	Fastqsanger.gz
	SRR26538070.fastq.gz	Fastqsanger.gz
	SRR26538071.fastq.gz	Fastqsanger.gz
	SRR26538072.fastq.gz	Fastqsanger.gz
	SRR26538073.fastq.gz	Fastqsanger.gz

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RNA-sequencing of Mtb H37Rv untreated and H37Rv treated with 2XMIC CJ067

Project description:The ability ofMycobacterium tuberculosis(Mtb) to tolerate nitric oxide (NO) and superoxide (O2•−) produced by phagocytes contributes to its success as a human pathogen. Recombination of NO and O2•− generates peroxynitrite (ONOO-), a potent oxidant produced inside immunologically-activated macrophages and causes lethality in diverse organisms. However, while the response ofMtbtowards NO and O2•− is well established, howMtbresponds to ONOO- remains uncertain. Filling this knowledge gap is important to understand the persistence mechanisms of Mtb during infection Hence, we used an endogenous peroxynitrite donor CJ067 and treated Mtb to identify new mechanisms of ONOO- resistance in mycobacteria.

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