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The single-molecule accessibility landscape of newly replicated mammalian chromatin

ABSTRACT: The single-molecule accessibility landscape of newly replicated mammalian chromatin

PROVIDER: PRJNA1032606 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26540900_subreads.fastq.gz	Fastqsanger.gz
	SRR26540801_subreads.fastq.gz	Fastqsanger.gz
	SRR26540901_subreads.fastq.gz	Fastqsanger.gz
	SRR26540802_subreads.fastq.gz	Fastqsanger.gz
	SRR26540902_subreads.fastq.gz	Fastqsanger.gz

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The single-molecule accessibility landscape of newly replicated mammalian chromatin

Project description:The higher-order structure of newly replicated (i.e. ‘nascent’) chromatin fibers remains poorly-resolved, limiting our understanding of how epigenomes are maintained across cell divisions. To address this, we present Replication-Aware Single-molecule Accessibility Mapping (RASAM), a long-read sequencing method that nondestructively measures genome-wide replication-status and protein-DNA interactions simultaneously on intact chromatin templates. We report that individual human and mouse nascent chromatin fibers are ‘hyperaccessible’ compared to steady-state chromatin. This hyperaccessibility occurs at two, coupled length-scales: first, individual nucleosome core particles on nascent DNA exist as a mixture of partially-unwrapped nucleosomes and other subnucleosomal species; second, newly-replicated chromatin fibers are significantly enriched for irregularly-spaced nucleosomes on individual DNA molecules. Focusing on specific cis-regulatory elements (e.g. transcription factor binding sites; active transcription start sites [TSSs]), we discover unique modes by which nascent chromatin hyperaccessibility is resolved at the single-molecule level: at CCCTC-binding factor (CTCF) binding sites, CTCF and nascent nucleosomes compete for motifs on nascent chromatin fibers, resulting in quantitatively-reduced CTCF occupancy and motif accessibility post-replication; at active TSSs, high levels of steady-state chromatin accessibility are preserved, implying that nucleosome free regions (NFRs) are rapidly re-established behind the fork. Our study introduces a new paradigm for studying higher-order chromatin fiber organization behind the replication fork. More broadly, we uncover a unique organization of newly replicated chromatin that must be reset by active processes, providing a substrate for epigenetic reprogramming.

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