Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1033402 | ENA |
REPOSITORIES: ENA
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Project description:We report the de novo assembled transcriptome of Y-organs from two intermolt and two pre-molt blue crabs. Data was obtained from RNAseq, assembled using Trinity, and differential expression was determined using DEseq2 in R.
2020-07-17 | GSE154560 | GEO
Project description:Metabolic processes and sexual maturation closely interact during the long-distance reproductive migration of many fish species to their spawning grounds. In the present study, we have for the first time used exercise experimentally to investigate the effects on sexual maturation in rainbow trout. Pubertal autumn-spawning seawater-raised female rainbow trout Oncorhynchus mykiss (n=26; 50-cm, 1.5-kg) were rested or swum at a near optimal speed of 0.75 body-lengths per second in a 6,000 L swim-flume under natural reproductive conditions (16 °C fresh-water, starvation, 8h-light:16h-dark photoperiod). Fish were sampled after arrival and subsequently after 10 days (resting or swimming 307 km) and 20 days (resting or swimming 636 km). Ovarian development was significantly reduced in the swimmers. Analysis of the expression of key factors in the reproductive axis included pituitary kiss1-receptor, lh and fsh and ovarian lh-receptor, fsh-receptor, aromatase and vitellogenin-receptor (vtgr). Swimmers had lower pituitary lh and ovarian vtgr expression than resters. Furthermore, the number of late vitellogenic oocytes was lower in swimmers than in resters, probably resulting from the lower vtgr expression, and vitellogenin plasma levels were higher. Therefore, swimming exercise suppresses oocyte development possibly by inhibiting vitellogenin uptake. Transcriptomic changes that occurred in the ovary of exercised fish were investigated using a salmonid cDNA microarray platform. Protein biosynthesis and energy provision were among the sixteen functional categories that were all down-regulated in the ovary. Down-regulation of the transcriptomic response in the ovary illustrates the priority of energy reallocation and will save energy to fuel exercise. A swimming-induced ovarian developmental suppression at the start of vitellogenesis during long-term reproductive migration may be a strategy to avoid precocious muscle atrophy.
2010-02-05 | GSE20131 | GEO
Project description:The decrease of pH level in the water affects animals living in aquatic habitat, such as crustaceans. The molecular mechanisms enabling these animals to survive this environmental stress remain unknown. To understand the modulatory function of neuropeptides in crustaceans when encountering drops in pH level, we developed and implemented a multifaceted mass spectrometric platform to investigate the global neuropeptide changes in response to water acidification in the Atlantic blue crab, Callinectes sapidus. Neural tissues were collected at different incubation periods to monitor dynamic changes of neuropeptides under different stress conditions occurring in the animal. Neuropeptide families were found to exhibit distinct expression patterns in different tissues and even each isoform had its specific response to the stress. Circulating fluid in the crabs (hemolymph) was also analyzed after 2-hour exposure to acidification condition, and together with results from tissue analysis, enabled the discovery of neuropeptides participating in the stress accommodation process as putative hormones. Two novel peptide sequences were detected in the hemolymph that appeared to be involved in the stress-related regulation in the crabs.
2021-09-08 | PXD013536 | Pride
Project description:Effects of ocean acidification on swimming crabs cultural water and gut bacterial communities
| PRJDB8309 | ENA
Project description:We report RNA sequencing data from 2 primary ovarian tumor, 2 omemtum and 1 normal ovary.
2021-11-19 | GSE181955 | GEO
Project description:Porcelain crabs, Petrolisthes cinctipes, live in the marine intertidal zone and routinely experience thermal stress. Genes involved in heat shock responses are generally upregulated following heat stress and genes involved in oxidative energy production are downregulated following heat stress We used microarrays to detail the global programme of gene expression underlying responses to thermal stress and identified distinct classes of up-regulated and down-regulated genes during this process. Keywords: time course Crabs were collected from the field and returned to the laboratory where they were given a heat stress or held under control conditions for the period of time during the heat stress. Samples from both heat stressed and control crabs were taken after crabs were placed into a common recovery tank for periods of time ranging from 0.5 to 30h
2007-04-16 | E-GEOD-7498 | biostudies-arrayexpress