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Profiling of murine tumors initiated by transplantation of engineered prostate organoids

ABSTRACT: Profiling of murine tumors initiated by transplantation of engineered prostate organoids

PROVIDER: PRJNA1034582 | ENA |

REPOSITORIES: ENA

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Bulk RNA-sequencing of mouse prostate tumors initiated by transplantation of engineered organoids.

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Project description:This SuperSeries is composed of the SubSeries listed below.

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Bulk RNA-sequencing of mouse prostate tumors initiated by transplantation of engineered organoids.

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Project description:Single-nuclear RNA-sequencing of murine organoid transplant-derived prostate tumors. Purpose: To identify differences in cell composition in murine prostatic tumors and to generate a reference dataset of all major cell types present in mouse prostate tumors Results: We recovered 4,872 cells with a median of 7055.5 UMIs per cell. Conclusions: snRNASeq reveals heterogeneity within neuroendocrine prostate cancer compartment, dominated by an Ascl1+ expression profile with mixed luminal lineage marker genes. snRNASeq recovers all the major epithelial, immune, and stromal cell types in prostate tumors. Spatial transcriptomic profiling of murine prostate tumors. Purpose: To spatially map the major cell types in the mouse prostatic tumors and identify spatially distinct neuroendocrine tumor compartments. Distinct tumor microenvironments were also used for identification of cell:cell signaling axes distributed between the histological subtypes. Conclusions: Spatial transcriptomic profiling of the mouse prostate reveals all major cell types and allows for cell:cell signaling analyses upon integration with our matching snRNA-Seq data

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