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Cell-type-specific translational regulation by human DUS enzymes [iCLIP]

ABSTRACT: Cell-type-specific translational regulation by human DUS enzymes [iCLIP]

PROVIDER: PRJNA1034648 | ENA |

REPOSITORIES: ENA

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Cell-type-specific translational regulation by human DUS enzymes [iCLIP]

Project description:Dihydrouridine is an abundant and evolutionary conserved modified nucleoside present on tRNA, but characterization and functional studies of individual modification sites and associated DUS writer enzymes in mammals is lacking. Here we use a chemical probing strategy, RNABPP-PS, to identify 5-chlorouridine as a general activity-based probe for human DUS enzymes. We map D modifications using mechanism-based RNA-protein crosslinking and also through chemical reactivity and mutational profiling to reveal the landscape of D modification sites on human tRNAs. Further, we knockout individual DUS genes in two model human cell lines to investigate their regulation of tRNA expression levels and codon-specific translation elongation. We show that whereas D modifications are present across most tRNA species, loss of D only perturbs the translational function of a subset of tRNAs in a cell-type-specific manner. Our work provides powerful chemical strategies for investigating D and DUS enzymes in diverse biological systems and provides insight into the role of a ubiquitous tRNA modification in translational regulation.

2024-10-31 | GSE246817 | GEO

Cell-type-specific translational regulation by human DUS enzymes

Project description:Cell-type-specific translational regulation by human DUS enzymes

| PRJNA1034645 | ENA

Cell-type-specific translational regulation by human DUS enzymes [tRNA NaBH4]

Project description:Cell-type-specific translational regulation by human DUS enzymes [tRNA NaBH4]

| PRJNA1034647 | ENA

Cell-type-specific translational regulation by human DUS enzymes [tRNA DESeq]

Project description:Cell-type-specific translational regulation by human DUS enzymes [tRNA DESeq]

| PRJNA1034643 | ENA

Cell-type-specific translational regulation by human DUS enzymes [tRNA NaBH4]

2024-10-31 | GSE246816 | GEO

Cell-type-specific translational regulation by human DUS enzymes [tRNA DESeq]

2024-10-31 | GSE246814 | GEO

Extensive allele-specific translational regulation

Project description:Extensive allele-specific translational regulation in hybrid mice

| PRJEB8233 | ENA

Cell-type-specific translational regulation by human DUS enzymes

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-10-31 | GSE246818 | GEO

Mapping of MLE and MSL2 using iCLIP

Project description:Dosage compensation in Drosophila is an epigenetic phenomenon utilizing proteins and long non-coding RNAs (lncRNAs) for transcriptional up-regulation of the entire X-chromosome. Here, UV cross-linking followed by deep sequencing (iCLIP) show that two enzymes in the Male-Specific Lethal complex, MLE RNA helicase and MSL2 ubiquitin ligase, bind evolutionarily conserved domains containing tandem stem-loops in roX1 and roX2 RNAs in vivo.

2013-07-18 | E-MTAB-1669 | biostudies-arrayexpress

Translational regulation of acetogenesis in Acetobacterium woodii

Project description:Translational regulation of acetogenesis in Acetobacterium woodii

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