Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Concentrations of 27 cytokines and chemokines in the culture medium of HUVECs

ABSTRACT: Concentrations of 27 cytokines and chemokines in the culture medium of HUVECs

PROVIDER: PRJNA1034996 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Concentrations of 27 cytokines and chemokines in the culture medium of HUVECs

Project description:Culture medium of HUVECs was collected and analyzed with the Bio-Plex Pro™ Human Cytokine 27-plex Panel.

2023-11-07 | GSE246852 | GEO

Cytokines & Chemokines PCR Array on human DC

Project description:Stimulation of human DC with LPS, VSL#3, or a combination of both resulted in their maturation, evident from enhanced expression of activation markers on the cell-surface, as well as the induction of various chemokines and cytokines.

2014-10-23 | GSE62583 | GEO

LPS induced Cytokines & Chemokines changes in E9.5 pregnant mouse uteri

Project description:Changes of Cytokines and chemokines profiles were sucessfully determined between mouse non-treated vs LPS treated pregnant uteri using Qiagen PCR array.

2015-01-01 | GSE63246 | GEO

LPS induced Cytokines & Chemokines changes in E9.5 pregnant mouse uteri

Project description:Changes of Cytokines and chemokines profiles were sucessfully determined between mouse non-treated vs LPS treated pregnant uteri using Qiagen PCR array. Pregnant mouse (FVB strain E8.5-9.5) were treated with LPS(2.5ug/mouse) or saline as control. 8h- or 16-h later, uteri tissues (N=3) were collected.

2015-01-01 | E-GEOD-63246 | biostudies-arrayexpress

Real-time quantitative PCR analysis of murine splenic chemokines and cytokines

Project description:Real-time quantitative PCR analysis of murine splenic chemokines and cytokines

| PRJNA328471 | ENA

ZIKV infection increases HUVECs endothelial barrier permeability and activates inflammatory cytokines

Project description:ZIKV infection increases HUVECs endothelial barrier permeability and activates inflammatory cytokines

| PRJNA488555 | ENA

Differential expression of mRNA encoding cytokines and chemokines in the reproductive tract after infection of mice with Chlamydia trachomatis

Project description:Chlamydia trachomatis is an obligate intracellular pathogen and the most frequently reported sexually transmitted bacteria in the United States. Infection with Chlamydia trachomatis targets epithelial cells within the genital tract which respond by secreting chemokines and cytokines. Persistent inflammation can lead to fibrosis, tubal infertility and/or ectopic pregnancy. Our objective was to determine the inflammatory mediators involved in clearance of low-grade infection and the potential involvement in chronic inflammation. Our hypothesis was that mRNA encoding pro-inflammatory cytokines and chemokines will be differentially expressed in the female reproductive tract of mice infected with C. trachomatis at both 28 and 35 days post-infection compared to controls. Superarray analysis was performed using RT2 Profiler PCR arrays for mouse Cytokines and Chemokines (Qiagen).

2014-10-18 | GSE62461 | GEO

Real-time quantitative PCR analysis of cytokines and chemokines in C57BL/6 mouse liver ischemic-reperfusion injury (IRI) model.

Project description:We assessed mouse cytokines and chemokines gene expression in fatty liver ischemic-reperfusion injury (IRI). C57BL/6 mice were fed with a normal chow diet (ND, 4.09 kcal/gram,13.4% kJ/fat) or a high-fat diet (HFD, 5.10 kcal/gram, 60% kJ/fat) and further grouped to treat with orally administered N-acetylcysteine (NAC, 10g/L). To induce liver ischemic-reperfusion injury in mice, an atraumatic micro clip was placed across the hepatic hilus, which interrupted the blood supply to the left and median lobes of the liver for 45-minutes partial warm ischemia time. After 24 hours of liver IRI, the affected left lobe of the liver was harvested and stored in Allprotect Tissue Reagent (Qiagen). We used Qiagen RT² Profiler™ PCR Array for mouse cytokines & chemokines to distinguish immunologically related gene signatures specific to the liver IRI in mice and characterize the effect of NAC treatment in HFD mice.

2022-07-30 | GSE209733 | GEO

Quantification of cytokines secreted by primary human cells using MRM: evaluation of analytical parameters – shotgun secretome data of untreated HUVECs

Project description:The determination of secreted proteins may provide highly valuable information about cell functions. While the typical methods for the determination of biologically relevant but low-abundant molecular species still relies on the use of specific antibodies, mass spectrometry-based methods are now gaining sufficient sensitivity to cope with such challenges. In the current study we have identified several cytokines and chemokines which were induced by inflammatory activation of primary human umbilical vein endothelial cells. Based on the high-resolution mass spectrometry data obtained with a Q Exactive orbitrap, we built an MRM method to quantify the most relevant molecules selected from the screening experiment. Using nano-flow Chip-HPLC coupled to a 6490 triple-quadrupole MS for MRM analyses we achieved calibration curves covering a linear range of four orders of magnitude and detection limits in the low attomol per microliter concentration range. Carryover was consistently less than 0.005%, the accuracy between 80% and 120% and the median coefficient of variation for LC/MS was only 2.2%. When including the variance introduced by biological replicates and the digestion procedure, the coefficient of variation was less than 20% for most peptides. Selection of appropriate marker molecules allowed us to monitor typical cell culture variations such as different cell densities, proliferative states and the occurrence of cell death. As a result, here we present a robust and efficient MRM-based assay for the accurate and sensitive determination of cytokines and chemokines representative for functional cell states and including comprehensive quality controls.

2015-06-29 | PXD002212 | Pride

Quantification of cytokines secreted by primary human cells using MRM: evaluation of analytical parameters – shotgun secretome data of inflammatory stimulated HUVECs

2015-06-29 | PXD002211 | Pride