Honey samples
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1035263 | ENA |
REPOSITORIES: ENA
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Project description:Honey promotes health and is an effective non-pharmacological home remedy against common respiratory infections. However, industrial processing and manipulation of raw honey can have a detrimental effect on its biological activities, including antibacterial ones, and hence its health-benefiting qualities. Therefore, this study aimed to compare the honey’s antibacterial activity, its total protein content, and the abundance of the most dominant bee-derived proteins in honey between raw (n=92) and supermarket (n=17) samples. We showed that raw honey samples were much more effective in inhibiting the growth of Staphylococcus aureus with a median minimal inhibitory concentration (MIC) value of 4.5% compared to supermarket honey samples ceasing bacterial growth with a median MIC value of 36%. Moreover, raw honey samples contained significantly higher amounts of total protein as well as the content of particular bee-derived proteins (major royal jelly protein 1 (MRJP1), glucose oxidase (GOX), and α-glucosidase) in contrast to supermarket honey samples. These data hint that some marketed honey samples could be deliberately manipulated with syrup, especially those that exhibited low protein content. In addition, the supermarket honey sample with the lowest protein content contained α-amylase (diastase) from Aspergillus oryzae. Strikingly, the content of this foreign enzyme in honey was roughly 60 times higher than the naturally occurring bee α-amylase. Our findings highlight the burning need to refine and monitor the specific quality parameters, ensuring the authenticity of honey and maintaining its reputation as a functional food.
2023-08-12 | PXD040190 | Pride
Project description:honey bee gut microbiome Raw sequence reads
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Project description:Transcriptome sequencing has become the main methodology for analyzing the relationship between genes and characteristics of interests, particularly those associated with diseases and economic traits. Because of its functional superiority, commercial royal jelly (RJ) and its production are major areas of focus in the field of apiculture. Multiple lines of evidence have demonstrated that many factors affect RJ output by activating or inhibiting various target genes and signaling pathways to augment their efficient replication. The coding sequences made available by the Honey Bee Genome Sequencing Consortium have permitted a pathway-based approach for investigating the development of the hypopharyngeal glands (HGs). In the present study, 3573941, 3562730, 3551541, 3524453, and 3615558 clean reads were obtained from the HGs of five full-sister honey bee samples using Solexa RNA sequencing technology. These reads were then assembled into 18378, 17785, 17065, 17105, and 17995 unigenes, respectively, and aligned to the DFCI Honey Bee Gene Index database. The differentially expressed genes (DEGs) data were also correlated with detailed morphological data for HGs acini. The results identify areas that warrant further study, including those that can be used to improve honey bee breeding techniques and help ensure stable yields of RJ with high quality traits.
2014-05-20 | GSE47136 | GEO
Project description:Freshwater samples (Beaches samples) Raw sequence reads
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