Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide chromatin profiles of ZNF395 [RNA-seq]

ABSTRACT: Genome-wide chromatin profiles of ZNF395 [RNA-seq]

PROVIDER: PRJNA1036244 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR26687660_1.fastq.gz	Fastqsanger.gz
	SRR26687660_2.fastq.gz	Fastqsanger.gz
	SRR26687661_1.fastq.gz	Fastqsanger.gz
	SRR26687661_2.fastq.gz	Fastqsanger.gz
	SRR26687662_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Genome-wide chromatin profiles of ZNF395

Project description:Genome-wide chromatin profiles of ZNF395

| PRJNA1036237 | ENA

Genome-wide chromatin profiles of ZNF395 [ChIP-seq]

Project description:Genome-wide chromatin profiles of ZNF395 [ChIP-seq]

| PRJNA1036243 | ENA

Genome-wide chromatin profiles of ZNF395 [RNA-seq]

Project description:Hypoxia signaling induced by VHL deficiency fuels growth but also poses immense metabolic stress to clear cell renal cell carcinomas (ccRCCs). Tumor cells depend on glutamine as the primary source of tricarboxylic acid (TCA) anaplerosis. Hypoxia-inducible factor alpha (HIFα) governs glycolysis but does not directly regulate glutamine metabolism; instead, the factor responsible for orchestrating glutamine metabolism and mitochondrial adaptations to hypoxia remains elusive. We now show that ZNF395 is a hypoxia-responsive factor that regulates glutamine metabolism in the mitochondria. ZNF395 is activated by a HIF2α-modulated super-enhancer but plays non-redundant functions from HIF2α. Specifically, ZNF395 facilitates the transcription of enzymes essential for glutaminolysis, including glutaminase (GLS) and isocitrate dehydrogenase 2 (IDH2). Functionally, ZNF395 depletion results in reduced TCA intermediates and their derivatives, including amino acids, glutathione and pyrimidine nucleotides. In addition, ZNF395 depletion diminishes mitochondrial respiration; restoration of complex I function rescues the effects of ZNF395 depletion. Our study underscores the coordinated role of HIFα and ZNF395 in shaping metabolic adaptations in response to hypoxia in VHL-deficient ccRCCs.

2024-10-01 | GSE247117 | GEO

Genome-wide chromatin profiles of PBRM1 [RNA-Seq]

Project description:Genome-wide chromatin profiles of PBRM1 [RNA-Seq]

| PRJNA640299 | ENA

Genome-wide chromatin profiles of ZNF395 [ChIP-seq]

2024-10-01 | GSE247116 | GEO

Genome-wide chromatin profiles of PBRM1

Project description:Genome-wide chromatin profiles of PBRM1

| PRJNA640292 | ENA

Genome-wide chromatin profiles of PBRM1 [ChIP-Seq]

Project description:Genome-wide chromatin profiles of PBRM1 [ChIP-Seq]

| PRJNA640096 | ENA

Genome-wide chromatin profiles of PBRM1 Drug treatment [RNA-Seq]

Project description:Genome-wide chromatin profiles of PBRM1 Drug treatment [RNA-Seq]

| PRJNA918666 | ENA

Genome-wide chromatin profiles of PBRM1 Drug treatment [ChIP-Seq]

Project description:Genome-wide chromatin profiles of PBRM1 Drug treatment [ChIP-Seq]

| PRJNA918665 | ENA

BANC-seq to identify genome-wide transcription factor binding affinities to native chromatin

Project description:BANC-seq (Binding Affinities to Native Chromatin by sequencing) allows determination of absolute apparent binding affinites of transcription factors to native chromatin in a genome-wide manner. In this study, we establish the method and show that chromatin and DNA sequence define binding affinites of FOXA1, SP1, YY1 and MYC/MAX complex. To relate the identified binding affinities to the actual transcriptional levels of these transcription factors in the cells used in this study, and to confirm that the nuclear isolation protocol used in the study does not lead to loss of nuclear proteins, we have generated whole proteomes including proteomics standards for absolute quantification of proteins. In addition, to validate some of the findings of the study, we have performed PAQMAN (Protein-nucleic acid affinity quantification by MAss spectrometry in nuclear extracts), as well as DNA pulldowns followed by mass spec.

2023-02-09 | PXD038502 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data