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Investigation of lncRNA expression profiles and analysis of immune-related lncRNA-miRNA-mRNA networks in neovascular age-related macular degeneration

ABSTRACT: Investigation of lncRNA expression profiles and analysis of immune-related lncRNA-miRNA-mRNA networks in neovascular age-related macular degeneration

PROVIDER: PRJNA1036560 | ENA |

REPOSITORIES: ENA

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Total blood monocyte gene expression from neovascular age-related macular degeneration patients and age-matched controls

Project description:Mononuclear phagocytes (MPs), including monocytes and macrophages, play complex roles in the pathogenesis of age-related macular degeneration (AMD). We aimed to perform global transcriptome analysis on monocytes from AMD patients to obtain additional insight to the role of MPs in AMD. Peripheral blood was taken from treatment-naïve neovascular AMD (nvAMD) patients (n=14), and age-matched controls (n=15). Peripheral blood mononuclear cells (PBMCs) were separated and monocytes were isolated via negative selection. Gene expression was evaluated with Affymetrix Gene1.0 ST microarrays. Statistical/bioinformatics analysis was performed using open sourceware programs.

2017-02-10 | GSE76237 | GEO

Impaired RPE Mitochondrial Energetics Leads Subretinal Fibrosis in Neovascular Age-related Macular Degeneration

Project description:Impaired RPE Mitochondrial Energetics Leads Subretinal Fibrosis in Neovascular Age-related Macular Degeneration

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Impaired RPE Mitochondrial Energetics Leads Subretinal Fibrosis in Neovascular Age-related Macular Degeneration

Project description:Subretinal fibrosis permanently impairs the vision of patients with neovascular age-related macular degeneration. Despite emerging evidence revealing the association between disturbed metabolism in retinal pigment epithelium (RPE) and subretinal fibrosis, the underlying mechanism remains unclear. In the present study, single-cell RNA sequencing revealed, prior to subretinal fibrosis, genes in mitochondrial fatty acid oxidation are downregulated in the RPE lacking very low-density lipoprotein receptor (VLDLR), especially the rate-limiting enzyme carnitine palmitoyltransferase 1A (CPT1A). We found that overexpression of CPT1A in the RPE of Vldlr-/- mice suppresses epithelial-to-mesenchymal transition and fibrosis. Mechanistically, TGFβ2 induces fibrosis by activating a Warburg-like effect, i.e. increased glycolysis and decreased mitochondrial respiration through ERK-dependent CPT1A degradation. Moreover, VLDLR blocks the formation of the TGFβ receptor I/II complex by interacting with unglycosylated TGFβ receptor II. In conclusion, VLDLR suppresses fibrosis by attenuating TGFβ2-induced metabolic reprogramming, and CPT1A is a potential target for treating subretinal fibrosis.

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Epigenetic Regulation of IL17RC in Age-related Macular Degeneration

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Investigation of lncRNA expression profiles and analysis of immune-related lncRNA-miRNA-mRNA networks in neovascular age-related macular degeneration

Project description:The aim of the present study was to investigate the alteration of lncRNAs and mRNAs in neovascular AMD. The differentially expressed mRNAs (DEmRNAs) and lncRNAs (DElncRNAs) were identified and the immune-related ceRNA network was characterized.

2023-11-07 | GSE247168 | GEO

Natural killer cells cooperate with neutrophils to suppress pathological angiogenesis in neovascular age-related macular degeneration

Project description:Natural killer cells cooperate with neutrophils to suppress pathological angiogenesis in neovascular age-related macular degeneration

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Transcriptomic Characterization of Human Choroidal Neovascular Membranes Identifies Calprotectin as a Novel Biomarker for Patients with Age-related Macular Degeneration

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Natural killer cells cooperate with neutrophils to suppress pathological angiogenesis in neovascular age-related macular degeneration

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2022-02-24 | GSE110623 | GEO

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Project description:Role of NR4A1 and non-classical monocytes in a mouse model of neovascular age-related macular degeneration

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