ABSTRACT: Whole genome sequence resources for Pseudomonas amygdali pv. sesami and Xanthomonas arboricola isolated from sesame (Sesamum indicum) in North Carolina in 2022
Project description:Traditional medicinal plants contain a variety of bioactive natural products including cysteine-rich (Cys-rich) antimicrobial peptides (AMPs). Cys-rich AMPs are often crosslinked by multiple disulfide bonds which increase their resistance to chemical and enzymatic degradation. However, this class of molecules is relatively underexplored. Herein, in silico analysis predicted 80 – 100 Cys-rich AMPs per species from three edible traditional medicinal plants: Linum usitatissimum (flax), Trifolium pratense (red clover), and Sesamum indicum (sesame). Proteomics analysis of seed peptide extracts revealed direct evidence for the translation of 3-10 Cys-rich AMPs per species including lipid transfer proteins, defensins, α-hairpinins, and snakins. Negative activity revealed by antibacterial screening highlights the importance of employing a multi-pronged approach for AMP discovery. Further, this study demonstrates that flax, red clover, and sesame are promising sources of further AMP discovery and characterization.
Project description:Sesamum indicum is an oilseed crop. One of the biotic factor that affect the production is a phyllody disease caused by phytoplasma which may target the reproductive system of the plant rendering the plant sterile. The aim of the study is to see the differential expression at proteomic level during the phytoplasma infection in Sesamum indicum.
