Project description:Purpose: We investigated root foraging strategies for K of tea plants using a multi-layer split-root system by RNA-seq. Methods: One-year old tea cuttings were cultivated with the roots evenly planted on the two sides of the split root hydroponic box with a root canal. Three treatments were included to simulate the heterogeneous and homogeneous K environments. After 5d treatment, the roots on the two sides of the split root hydroponic box were collected separately and the RNA sequencing were analyzed by the Illumina Hiseq (2500, Illumina, San Diego, CA). Results: RNA-seq data had a linear relationship with qRT–PCR (r2=0.76), which confirmed the reliability of the RNA-seq data. Conclusions: Our study screened the key genes of tea root system to adapt to potassium heterogeneity.
Project description:We used a transcriptome sequencing approach to analyze different expression levels of three barley varieties under both infected and uninfected conditions
Project description:To identify the putative genes involved in theacrine biosynthesis in tea plant, we carried out comparative transcriptome analysis of Kucha (K6 and K11) and conventional varieties (YH 9 and QX 1).
Project description:To aid the annotation of the genome of cultivated (ZSZ1) and wild (QYZM) ramie and characterize the expression profiling, the root, stem and leaves from these two varieties were performed for transcriptome sequencing. Our results generated 20,693 and 19,498 predicted genes from QYZM and ZSZ1 genome. of them, 17,983 QYZM genes and 17,998 ZSZ1 expressed in at least one of these three examined tissues.
