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RNA-seq of osteogenesis following siRNA depletion of SERPINA3

ABSTRACT: RNA-seq of osteogenesis following siRNA depletion of SERPINA3

PROVIDER: PRJNA1039295 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26792300.fastq.gz	Fastqsanger.gz
	SRR26792301.fastq.gz	Fastqsanger.gz
	SRR26792302.fastq.gz	Fastqsanger.gz
	SRR26792303.fastq.gz	Fastqsanger.gz
	SRR26792304.fastq.gz	Fastqsanger.gz

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RNA-seq of osteogenesis following siRNA depletion of SERPINA3

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Osteogenesis RNA-Seq

Project description:Bone development and repair depends on the differentiation of mesenchymal stem cells (MSCs) into osteoblasts. MSCs can be differentiated towards osteoblasts in vitro, making these cells a convenient tool for investigation of osteogenesis. Molecular characterization of this process is relevant for the application of MSCs in skeletal regenerative medicine, and for understanding the deregulation of osteogenesis in bone disease. Cellular differentiation is driven by highly regulated changes in gene expression, which at the level of transcription is associated with DNA binding of transcriptional regulators and local changes in chromatin landscape. By sequencing of RNA (RNA-Seq) and immunoprecipitated chromatin (ChIP-Seq) we have characterized gene expression, histone modification changes and DNA binding of the bone master regulator RUNX2 in osteogenic differentiation. Data from the RNA-Seq experiment has also been deposited at ArrayExpress under accession number E-MTAB-1829 (https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1829/).

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