Project description:Gene expression programs depend on sequence-specific DNA binding transcription factors, but the mechanisms that control the selective binding of these factors in a chromosomal and genomic context remain enigmatic. Here, we show that two master regulators of B-cell fate, namely EBF1 and RBP-jk, show variable genome-wide chromosome distribution in two related B-lymphocyte lines carrying different forms of Epstein-Barr Virus (EBV) latency. The latency-type specific EBV-encoded EBNA2 colocalized with RBP-jk and EBF1 at induced binding sites. Colocalization of EBF1, RBP-jk, and EBNA2 correlated with transcriptional activation. Conditional expression or repression of EBNA2 lead to a rapid alteration in RBP-jk and EBF1 binding. Biochemical and shRNA depletion studies provide evidence for cooperative assembly at co-occupied sites. These findings reveal that non-DNA binding cofactors can facilitate combinatorial interactions to induce new patterns of transcription factor occupancy and gene programming Examination of EBNA2/EBF1/EBP-jk binding in MutuI and LCL cell lines

2015-12-16 | E-GEOD-75503 | biostudies-arrayexpress

Bartonella quintana JK 67 strain:JK 67

Project description:Bartonella quintana JK 67 strain:JK 67 Genome sequencing and assembly

| PRJNA81677 | ENA

Bartonella quintana JK 63 strain:JK 63

Project description:Bartonella quintana JK 63 strain:JK 63 Genome sequencing and assembly

| PRJNA81683 | ENA

Bartonella henselae JK 53 strain:JK 53

Project description:Bartonella henselae JK 53 strain:JK 53 Genome sequencing and assembly

| PRJNA81689 | ENA

Bartonella quintana JK 19 strain:JK 19

Project description:Bartonella quintana JK 19 strain:JK 19 Genome sequencing and assembly