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Transcriptional changes on lymphatic endothelial cells upon co-culture with human melanoma cells

ABSTRACT: Transcriptional changes on lymphatic endothelial cells upon co-culture with human melanoma cells

PROVIDER: PRJNA1039499 | ENA |

REPOSITORIES: ENA

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Transcriptional changes on lymphatic endothelial cells upon co-culture with human melanoma cells

Project description:The aim was to study transcriptional changes on the primary human lymphatic endothelial cells when they have been in contact with a human melanoma cell line

2023-11-20 | GSE247542 | GEO

RNA profiling of human lymphatic endothelial cells exposed to melanoma exosomes

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2021-07-16 | GSE135187 | GEO

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Transcriptional profiling of melanoma-associated lymphatic endothelial cells (LECs)

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Metabolomics revealed the influence of breast cancer on lymphatic endothelial cell metabolism, metabolic crosstalk, and lymphangiogenic signaling in co-culture

Project description:<p>Breast cancer metastasis occurs via blood and lymphatic vessels. Breast cancer cells 'educate' lymphatic endothelial cells (LECs) to support tumor vascularization and growth. However, despite known metabolic alterations in breast cancer, it remains unclear how lymphatic endothelial cell metabolism is altered in the tumor microenvironment and its effect in lymphangiogenic signaling in LECs. We analyzed metabolites inside LECs in co-culture with MCF-7, MDA-MB-231, and SK-BR-3 breast cancer cell lines using 1H nuclear magnetic resonance (NMR) metabolomics, Seahorse, and the spatial distribution of metabolic co-enzymes using optical redox ratio imaging to describe breast cancer-LEC metabolic crosstalk. LECs co-cultured with breast cancer cells exhibited cell-line dependent altered metabolic profiles, including significant changes in lactate concentration in breast cancer co-culture. Cell metabolic phenotype analysis using Seahorse showed LECs in co-culture exhibited reduced mitochondrial respiration, increased reliance on glycolysis and reduced metabolic flexibility. Optical redox ratio measurements revealed reduced NAD(P)H levels in LECs potentially due to increased NAD(P)H utilization to maintain redox homeostasis. 13C-labeled glucose experiments did not reveal lactate shuttling into LECs from breast cancer cells, yet showed other 13C signals in LECs suggesting internalized metabolites and metabolic exchange between the two cell types. We also determined that breast cancer co-culture stimulated lymphangiogenic signaling in LECs, yet activation was not stimulated by lactate alone. Increased lymphangiogenic signaling suggests paracrine signaling between LECs and breast cancer cells which could have a pro-metastatic role.</p>

2024-06-25 | MTBLS859 | MetaboLights

RNA profiling of human lymphatic endothelial cells exposed to melanoma exosomes

Project description:RNA profiling of human lymphatic endothelial cells exposed to melanoma exosomes

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Project description:dsRNA mimics treatment of Melanoma and Human lymphatic cells

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Transcriptome analysis upon lymphatic endothelial cells (LEC) and cancer cells (Haca4) co-culture

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2023-04-21 | GSE227697 | GEO

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