Project description:Transcriptional profiling of the bacteria Paenibacillus vortex comparing control untreated cells with kanamycin treated cells after 18 hours of exposure. Goal was to determine the effect of the antibiotic kanamycin in concentration which affect the colony morphology on global bacteria gene expression.
Project description:Here we apply a metabolomics to study the interaction of two co-isolated model organisms, Xanthomonas retroflexus and Paenibacillus amylolyticus. When cultured in close proximity on agar plates Xanthomonas retroflexus induces a clear morphological response from Paenibacillus amylolyticus, leading to enhanced growth of Paenibacillus amylolyticus. Application of 2D spatial metabolomics shows how Xanthomonas retroflexus influences the chemical environment by production specific metabolites.
Project description:We compare the transcriptome of gnotobiotic Ae. aegypti generated by contaminating axenic (bacteria-free) larvae with bacterial isolates found in natural mosquito breeding sites. We focused on four bacterial isolates (Lysobacter, Flavobacterium, Paenibacillus and Enterobacteriaceae) and found that different gnotobiotic treatments resulted in massive transcriptomic changes throughout the mosquito development.