Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR26837304_1.fastq.gz | Fastqsanger.gz | |||
| SRR26837304_2.fastq.gz | Fastqsanger.gz | |||
| SRR26837305_1.fastq.gz | Fastqsanger.gz | |||
| SRR26837305_2.fastq.gz | Fastqsanger.gz | |||
| SRR26837306_1.fastq.gz | Fastqsanger.gz |
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Project description:This study investigates the effects of non-thermal levels of 900 MHz radiofrequency electromagnetic field (RF-EMF) which is used in mobile phone technology on neurodevelopment and neural stem cell differentiation. In vivo in the rat, the effects of pre- and post-natal 0.08 and 0.4 W/kg exposure were assessed on the proteomic profile at PND postnatal day 0 (PND) and on proliferation, synaptogenesis, and oxidative stress in the hippocampus and primary motor cortex of rats at PND 8 and PND 17. Complementary Mechanistic endpoints on cell differentiation were studied in vitro using stem cells exposed to 900 MHz RF-EMF at a non-thermal level for 3 or 7 days in vitro at the lowest SAR (0.08 W/kg). Our in vivo results showed a decrease in BDNF level and cells proliferation with a decrease in synapses balance. Increased proliferation, apoptosis, and double-strand DNA breaks in neural stem cells (NSCs) were also observed. Smaller ratio of B1 cells and a higher ratio of oligodendrocyte progenitor cells and astrocytes were observed in the exposed NSPCs. These findings suggest that developmental RF-EMF exposure induce changes in neurodevelopment in vivo from PND 0 and until PND 17 specifically on cellular proliferation supported by alterations in NSPCs in vitro, leading to the hypothesis of a vulnerability of developing central nervous system towards RF-EMF exposures at regulatory thresholds.
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Project description:Postoperative neurocognitive disorder (PND) is one of the most common postoperative neurological complications in aged patients. In order to detect the differential expression profiles of genes caused by PND, a total of 26 18-month-old male C57BL/6 mice were randomly assigned to control group and PND group. Behavioral tests showed that mice in the PND group had impaired cognitive function compared with the control group. Three mice in each group were randomly selected to harvest the brain for analysis the expressions of circRNAs, miRNAs and mRNAs in the prefrontal cortex by next-generation sequencing (NGS) technology. Differentially expressed genes, including 1192 circRNAs, 27 miRNAs and 266 mRNAs were identified, and its accuracy was further confirmed by qRT-PCR.
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Project description:Postoperative neurocognitive disorder (PND) is one of the most common postoperative neurological complications in aged patients. In order to detect the differential expression profiles of genes caused by PND, a total of 26 18-month-old male C57BL/6 mice were randomly assigned to control group and PND group. Behavioral tests showed that mice in the PND group had impaired cognitive function compared with the control group. Three mice in each group were randomly selected to harvest the brain for analysis the expressions of circRNAs, miRNAs and mRNAs in the prefrontal cortex by next-generation sequencing (NGS) technology. Differentially expressed genes, including 1192 circRNAs, 27 miRNAs and 266 mRNAs were identified, and its accuracy was further confirmed by qRT-PCR.
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Project description:Perioperative neurocognitive dysfunction (PND) is emerging as a significant complication of surgery in elderly patients. However, the molecular mechanisms of PND are not well known. Here we applied microarray analysis to identify circular RNAs (circRNAs) in hippocampus from a mouse model of PND and control mice. Then, the dysregulated circRNAs were confirmed via quantitative real-time polymerase chain reaction (qRT-PCR). Then, Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to probe the vital functions of dysregulated genes.
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Project description:The Göttingen Minipig is gaining ground as nonrodent species in safety testing of drugs for pediatric indications. Due to developmental changes in pharmacokinetics (PK) and pharmacodynamics (PD), physiologically-based pharmacokinetic (PBPK) models are built to better predict drug exposure in children and to aid species selection for nonclinical safety studies. These PBPK models require high quality physiological and PK/PD data such as protein abundance of drug metabolizing enzymes. These data are available for man and rat, but scarce for the Göttingen Minipig. The aim of this study was to assess hepatic cytochrome P450 (CYP) protein abundance in the developing Göttingen Minipig by using mass spectrometry. In addition, sex-related differences in CYP protein abundance and correlation of CYP enzyme activity with CYP protein abundance were assessed. The following age groups were included: gestational day (GD) 84 - 86 (n = 8), GD 108 (n = 6), postnatal day (PND) 1 (n = 8), PND 3 (n = 8), PND 7 (n = 8), PND 28 (n = 8) and adult (n = 8). Liver microsomes were extracted and protein abundance was compared to that in adult animals. Next, the CYP protein abundance was correlated to CYP enzyme activity in the same biological samples. In general, CYP protein abundance gradually increased during development. However, we observed a stable protein expression over time for CYP4A24 and CYP20A1 and for CYP51A1, a high protein expression during the fetal stages was followed by a decrease during the first month of life and an increase towards adulthood. Sex-related differences were observed for CYP4V2_2a and CYP20A1 at PND 1 with highest expression in females for both isoforms. In the adult samples, sex-related differences were detected for CYP1A1, CYP1A2, CYP2A19, CYP2E1_2, CYP3A22, CYP4V2_2a and CYP4V2_2b with higher values in female compared to male Göttingen Minipigs. The correlation analysis between CYP protein abundance and CYP enzyme activity showed that CYP3A22 protein abundance correlated clearly with the metabolism of midazolam at PND 7. These data are remarkably comparable to human data and provide a valuable step forward in the construction of a neonatal and juvenile Göttingen Minipig PBPK model.
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