Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1041308 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR26848114_1.fastq.gz | Fastqsanger.gz | |||
| SRR26848114_2.fastq.gz | Fastqsanger.gz | |||
| SRR26848115_1.fastq.gz | Fastqsanger.gz | |||
| SRR26848115_2.fastq.gz | Fastqsanger.gz | |||
| SRR26848116_1.fastq.gz | Fastqsanger.gz |
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Project description:The targeting range of CRISPR-Cas9 base editors (BEs) is limited by their G/C-rich PAM sequences. To overcome this limitation, we developed a CRISPR/Cpf1-based BE by fusing the rat cytosine deaminase APOBEC1 to a catalytically inactive version of Lachnospiraceae bacterium Cpf1. The base editor recognizes a T-rich PAM sequence and converts C to T in human cells with low levels of indels, non-C-to-T substitutions and off-target editing.
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