Project description:This SuperSeries is composed of the following subset Series: GSE5865: B.thetaiotaomicron or B. longum mono-association versus B. thetaiotaomicron/B. longum co-colonization, PR chow, NMRI GSE5866: B.thetaiotaomicron mono-association versus B. thetaiotaomicron co-colonization with B. longum, PR chow, B6 GSE5867: B.thetaiotaomicron mono-association versus B. thetaiotaomicron co-colonization with B. animalis or L. casei, PR chow, B6 Refer to individual Series
Project description:Transcriptional profiling of Bacteroides thetaiotaomicron was performed on samples taken from (i) chemostat cultures (in vitro) and (ii) from the ceca of gnotobiotic monoassociated NMRI mice (in vivo). In vitro profiles were obtained from biological duplicate cultures taken at five timepoints from log to stationary phase in three types of media: (i) minimal media glucose (MM-G), (ii) minimal media maltotriose (MM-M), and (iii) rich media (TYG). In vivo profiling was performed on 12 mice, each colonized with B. thetaiotaomicron for ten days. The in vivo profiling was conducted in two separate experiments: (i) 6 male NMRI mice fed standard polysaccharide rich mouse chow and (ii) 3 male NMRI mice fed standard polysaccharide rich mouse chow and 3 male NMRI mice fed a simple sugar diet containing glucose and sucrose but deficient in polysaccharides. Keywords: other
Project description:Genome wide transcriptional comparison of B. thetaiotaomicron wild type vs. an isogenic mutant (BT2923::pGERM). Cecal populations of wt or BT2923::pGERM monoassociated 10d with B6 or NMRI gnotobiotic mice fed a standard-chow polysaccharide rich (PR) diet were profiled. Keywords: wild type versus knockout, in vivo
