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Persistent alterations in glucose metabolism, gut microbiome, and adipose tissue transcriptome after two-week sleep recovery in chronically sleep-fragmented female mice

ABSTRACT: Persistent alterations in glucose metabolism, gut microbiome, and adipose tissue transcriptome after two-week sleep recovery in chronically sleep-fragmented female mice

PROVIDER: PRJNA1042630 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26872278_1.fastq.gz	Fastqsanger.gz
	SRR26872278_2.fastq.gz	Fastqsanger.gz
	SRR26872279_1.fastq.gz	Fastqsanger.gz
	SRR26872279_2.fastq.gz	Fastqsanger.gz
	SRR26872280_1.fastq.gz	Fastqsanger.gz

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Project description:Although the specific functions of sleep have not been completely elucidated, the literature has suggested that sleep is essential for proper homeostasis. Sleep loss is associated with changes in behavioral, neurochemical, cellular, and metabolic functions as well as impaired immune response. We evaluated the gene expression profiles of healthy volunteers submitted to 48 hours of prolonged wakefulness (PW) followed by 12 hours of sleep recovery (SR) using high-resolution microarrays. Peripheral whole blood was collected in the morning before the initiation of sleep deprivation (baseline), after the second night of PW, and one night after SR. the identified differentially expressed genes were related to immune response, DNA damage and repair as well as inflammation. Examples of these include: killer cell lectin-like receptors family granzymes and T-cell receptors, which play important roles in host defense. These results support the idea that sleep loss can lead to alteration of molecular processes that drive perturbation of cellular immunity, induction of inflammatory response and homeostatic imbalance. Moreover, down-regulation of multiple genes after prolonged wakefulness (in comparison with baseline condition) and up-regulated after sleep recovery (in comparison with prolonged wakefulness condition) were observed, suggesting an attempt of the body to re-establish internal homeostasis. In silico validation of alterations in the expression of CETN3, DNAJC, IGFR2B and CEACAM genes, confirmed the previous findings related to the molecular effects of sleep deprivation. It is clear that confirmatory studies will be necessary to fully validate the potential candidate genes and functional networks identified. Nevertheless, the present findings confirm that the effects of sleep deprivation are not restricted to the brain and can occur intensely in peripheral tissues. The peripheral blood from each volunteer (nine individuals) were collected in the baseline night and every morning after PW and after the night of SR.

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Persistent alterations in glucose metabolism, gut microbiome, and adipose tissue transcriptome after two-week sleep recovery in chronically sleep-fragmented female mice

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