Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1043430 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Investigation of whole genome gene expression level changes in rumen epithelium of dairy cattle at different stages of rumen development and on different diets.
2009-11-01 | GSE18382 | GEO
Project description:The rumen harbors a complex mixture of archaea, bacteria, protozoa and fungi that efficiently breakdown plant biomass and its complex dietary carbohydrates into soluble sugars that can be fermented and subsequently converted into metabolites and nutrients utilized by the host animal. While rumen bacteria populations have been well documented, only a fraction of the rumen eukarya are taxonomically and functionally characterized, despite the recognition that they contribute to the cellulolytic phenotype of the rumen fauna. To investigate how anaerobic fungi actively engage in digestion of recalcitrant fiber that is resistant to the initial stages of rumination, we resolved genome-centric metaproteome and metatranscriptome datasets generated from switchgrass samples incubated in nylon bags within the rumen of cannulated dairy cows for 48 hours.
2020-10-14 | PXD017007 | Pride
Project description:The purpose of this study was to determine the effects of normal diet feed (NF) and alternative diet feed (AF) on animal performance, gene expression in adipose, liver, and muscle, and changes in bacteria and fungi in the rumen of Bos-Taurus using high-throughput sequencing methods. In addition, Interactions between differentially expressed genes (DEGs) in major metabolic organs and rumen bacteria /fungi were studied. A total of 34,360 genes were found to be expressed across all tissues examined based on transcriptome analysis. According to our findings, 34, 36, 28 genes were differentially expressed in the adipose, liver, and muscle tissues, respectively. A majority of DEGs identified were related to osteoclast differentiation, phagosomes, and immune-functions etc. A study of rumen samples revealed that Firmicutes and Bacterioidetes were the most common phyla. An AF diet significantly increased Firmicutes abundance and reduced Bacterioidetes abundance (p< 0.05). Genus-level analysis revealed that the occurrence of Faecalicatena, Intestinimonas, Lachnoclostridium, Faecalicatena, and Intestinimonas was higher (p < 0.05) in animals fed with the AF diet than in animals fed with an NF diet. As for fungi, Neocallimastigomycota accounted for 98.2% of the NF diet and 86.88% of the AF diet. The AF increased the abundance of Orpinomyces (21.15% to 29.7%), Piromyces (0.1% to 1.8%), and other fungi, but reduced the abundance of Neocallimastix (72.0% to 25.2%). Analysis of the correlation between DEGs and microbes showed that rumen bacteria/fungi significantly influenced expression levels of genes in adipose, liver, and muscle tissues
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Project description:Comparison of gene expression in wild type and Wt1 (Wilms tumor suppressor gene 1) heterozygous knockout mice. Background: in Wt1+/- mice (MF1 strain) litter sizes are significantly smaller than in wild type animals. In addition, we observe retarded embryonic development of pre-implantation embryos that are still within the oviduct. By gene expression analysis we want to elucidate the molecular basis for these phenomena.
2015-12-12 | GSE75924 | GEO
Project description:In order to investigate the diurnal oscillations of ruminal bacteria, and their responses to the changes in different feeding patterns, we conducted an animal experiment by feeding the sheep ad libitum with a hay-based diet (50% of alfalfa hay and 46% of oats hay) and a grain-based diet (45% of corn meal and 11% of soybean meal) for 30 days, and ruminal fluid samples were collected at six different timepoints from T2 to T22 in one day, and the composition and diversity of the bacterial communities in rumen microbiomes of the sheep in the Grain-diet and Hay-diet groups at different timepoints were analyzed through 16S rRNA sequencing.
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