Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA104455 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:This SuperSeries is composed of the following subset Series: GSE4919: Translation State Array Analysis of Thrombin Stimulated Human Endothelial Cells GSE5352: Translation State Array Analysis of LPS Stimulated Human Endothelial Cells GSE5353: Translation State Array Analysis of Insulin Stimulated Human Endothelial Cells GSE5354: Translation State Array Analysis of TNF Stimulated Human Endothelial Cells GSE5385: Translation State Array Analysis of Histamine Stimulated Human Endothelial Cells GSE5386: Translation State Array Analysis of Serum Stimulated Human Endothelial Cells Refer to individual Series
2010-07-01 | E-GEOD-5393 | biostudies-arrayexpress
Project description:Translation State Array Analysis of LPS Stimulated Human Endothelial Cells
| PRJNA104313 | ENA
Project description:Translation State Array Analysis of Insulin Stimulated Human Endothelial Cells
| PRJNA104315 | ENA
Project description:Translation State Array Analysis of Histamine Stimulated Human Endothelial Cells
| PRJNA104321 | ENA
Project description:Translation State Array Analysis of Serum Stimulated Human Endothelial Cells
| PRJNA104323 | ENA
Project description:Translation State Array Analysis of TNF Stimulated Human Endothelial Cells
| PRJNA104317 | ENA
Project description:Confluent human umbilical vein endothelial cells (HUVECs) were exposed to Thrombin (2 U/mL) for 2 hours. Ribosomal profiling via gradient centrifugation and fractionation was used to separate monosome, or under-translated, and polysome, or actively translated, mRNA species that were then used to probe cDNA arrays, a process known as Translation State Array Analysis (TSAA). Four samples were obtained from these experiments, Control Monosome, Control Polysome, Thrombin Monosome, and Thrombin Polysome. Using the normalized signal intensities from the GeneFilters, we calculated a translation index, or measure of movement of an mRNA molecule from the monosome to the polysome fraction upon stimulation. This calculation was made as follows: (thrombin polysome/thrombin monosome)/(control polysome/control monosome). Translational indices greater than 2.5 (upregulated) or lower than 0.4 (downregulated) were chosen for further study. TSAA data suggests that JunB is translationally regulated by thrombin stimulation. Immunocytochemistry, western blotting and RT-PCR were used to verify the results of TSAA. Keywords: Translation State Array Analysis
2006-05-31 | GSE4919 | GEO
Project description:Transcription profiling by array of human umbilical vein endothelial cells (HUVEC) stimulated with TNF vs. controls reveals TNF induces distinct expression programs
2025-01-24 | E-GEOD-2639 | ExpressionAtlas
Project description:Transcription profiling by array of human umbilical vein endothelial cells expressing miR-17
2025-01-24 | E-GEOD-20745 | ExpressionAtlas
Project description:Thrombin exerts pleiotropic effects on the endothelium. Nevertheless, little is known about its capacity to generate endothelial microparticles, a hallmark of endothelial activation. To gain insight into the mechanisms involved in microparticles release, the human microvascular endothelial cell line-1 (HMEC1) was stimulated with thrombin at 1 IU/ml for different times. The gene expression profiling in stimulated HMEC-1 was compared to that obtain with untreated cells.
2006-01-20 | E-MEXP-477 | biostudies-arrayexpress