Project description:HT-29-MTX cells were treated with Ancylostoma ceylanicum hookworm larvae or left untreated. The differences in gene expression between treated and untreated samples was observed.
Project description:Parasitic infections with the hookworms Ancylostoma ceylanicum and Necator americanus are a significant global health burden and current hookworm infection management approaches are limited by modest drug efficacy, costs, risk of reinfection and drug resistance. Subunit vaccines based on proteins excreted and secreted (ES) by hookworms that impart sufficient efficacy to reduce worm numbers and associated disease burden is a promising management strategy to overcome these limitations. However, existing studies on the ES proteome of hookworms have mainly described proteins derived from the adult life stage which may preclude the opportunity to target larvae-specific parasitic processes. In this project, we use high resolution mass spectrometry to identify and compare ES proteins from the L3 stage as well as the adult stage of N. americanus and A. ceylanicum respectively.
Project description:Proteomic profiling of the excreted / secreted products from adult female and male hookworm Ancylostoma ceylanicum, including a media-only control sample.
