Project description:The associated files are mass spec data ........ of proteins from colonies of Saccharomyces cerevisiae BY4742, BY-rtg1, BY-mks1, BY-rtg1mks1 and BY-rtg2mks1 strains.
Project description:The goal of this study was to identify genes that are differentially expressed after genetic deletion of both alleles of the Cyp26a1 gene in murine embryonic stem cells. Cyp26a1 codes for the CYP26A1 enzyme which metabolizes RA to polar RA metabolites, such as 4-oxo-RA and 4-OH-RA. CYP26A1-/- ES cells do not metabolize RA within 48 hours of RA treatment while in Wt ES cells, polar RA metabolites are already detectable by 8 hr. In addition, the absence of CYP26A1 enzyme increases intracellular RA levels. By gene microarray analysis, we wanted to identify genes that would be affected by the lack of the Cyp26a1 gene. Experiment Overall Design: Wt and CYP26A1-/- ES cells were treated with control+LIF for 8 hours, and this was repeated 3 times. Wt and CYP26A1-/- ES cells were treated with 100 nM RA +LIF for 8 hours, and this was repeated 3 times. Wt and CYP26A1-/- ES cells were treated with 100 nM RA +LIF for 72 hours, and this was repeated 2 times.