Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1045847 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The response of soil microbial community to climate warming through both function shift and composition reorganization may profoundly influence global nutrient cycles, leading to potential significant carbon release from the terrain to the atmosphere. Despite the observed carbon flux change in northern permafrost, it remains unclear how soil microbial community contributes to this ecosystem alteration. Here, we applied microarray-based GeoChip 4.0 to investigate the functional and compositional response of subsurface (15~25cm) soil microbial community under about one year’s artificial heating (+2°C) in the Carbon in Permafrost Experimental Heating Research site on Alaska’s moist acidic tundra. Statistical analyses of GeoChip signal intensities showed significant microbial function shift in AK samples. Detrended correspondence analysis and dissimilarity tests (MRPP and ANOSIM) indicated significant functional structure difference between the warmed and the control communities. ANOVA revealed that 60% of the 70 detected individual genes in carbon, nitrogen, phosphorous and sulfur cyclings were substantially increased (p<0.05) by heating. 18 out of 33 detected carbon degradation genes were more abundant in warming samples in AK site, regardless of the discrepancy of labile or recalcitrant C, indicating a high temperature sensitivity of carbon degradation genes in rich carbon pool environment. These results demonstrated a rapid response of northern permafrost soil microbial community to warming. Considering the large carbon storage in northern permafrost region, microbial activity in this region may cause dramatic positive feedback to climate change, which is important and necessary to be integrated into climate change models.
2016-02-22 | GSE77866 | GEO
Project description:Interventions: Body temperature Protection group (T group):Intraoperative routine temperature protection+Inflatable heating system;Control group (C Group):Intraoperative routine temperature protection
Primary outcome(s): interleukin 17 (IL17);transforming growth factor-ß (TGF-ß);Interleukin- 6 (IL-6);Intraoperative core body temperature;Incidence of postoperative complications;Lymphocyte count
Study Design: Parallel
| 2699857 | ecrin-mdr-crc
Project description:Soil transplant serves as a proxy to simulate climate change in realistic climate regimes. Here, we assessed the effects of climate warming and cooling on soil microbial communities, which are key drivers in Earth’s biogeochemical cycles, four years after soil transplant over large transects from northern (N site) to central (NC site) and southern China (NS site) and vice versa. Four years after soil transplant, soil nitrogen components, microbial biomass, community phylogenetic and functional structures were altered. Microbial functional diversity, measured by a metagenomic tool named GeoChip, and phylogenetic diversity are increased with temperature, while microbial biomass were similar or decreased. Nevertheless, the effects of climate change was overridden by maize cropping, underscoring the need to disentangle them in research. Mantel tests and canonical correspondence analysis (CCA) demonstrated that vegetation, climatic factors (e.g., temperature and precipitation), soil nitrogen components and CO2 efflux were significantly correlated to the microbial community composition. Further investigation unveiled strong correlations between carbon cycling genes and CO2 efflux in bare soil but not cropped soil, and between nitrogen cycling genes and nitrification, which provides mechanistic understanding of these microbe-mediated processes and empowers an interesting possibility of incorporating bacterial gene abundance in greenhouse gas emission modeling.
2013-10-30 | GSE51592 | GEO
Project description:To further understand the gene expression characteristics of Pseudomonas aeruginosa PAO1, we have applied whole genome microarray expression profiling as a discovery platform to specify the temperature dependent expression of PAO1 genome at soil and human body temperature. We selected 28°C as temperature representative of the soil niche and 37°C for human body. The results from the temperature dependent transcriptome analysis are consistent to our previous published data that the phzM, ptsP and lasI genes expression is upregulated at 37°C [11]. The comparison analysis of the M18 genome expressional profiles at 28°C and 37°C indicated a total of 596 genes expressed in a temperature dependent manner over two fold.
2012-03-01 | GSE36161 | GEO
Project description:Plant roots located in the upper soil layers are prone to experience high temperatures. To gain insight into the effect of high temperature on root development and functioning, we exposed five-day-old Arabidopsis thaliana seedlings grown on agar plates to 30 °C for 48 hours, and compared the gene expression profile in the root tip with that from seedlings that remained at 22 °C.
2024-02-07 | GSE254973 | GEO
Project description:The increasing ambient temperature significantly impacts plant growth, development, and reproduction. Uncovering the temperature-regulating mechanisms in plants is of high importance, not only for boosting our plant biology knowledge but also for assisting plant breeders in improving plant resilience to these stress conditions. Numerous studies on the molecular mechanisms by which plants regulate temperature responses revealed that plants employ distinct transcription factors to regulate thermomorphogenesis specific to each tissue type. A significant discovery in this field was the identification of PHYTOCHROME-INTERACTING FACTORs (PIFs) as key regulators of thermomorphogenesis during vegetative growth. PIF4, a regulator of auxin-mediated signaling pathways, is crucial in controlling high-temperature responses. In this study, we screened the temperature responses of the wild type and several PhyB-PIF4 pathway Arabidopsis mutant lines in combined and integrative phenotyping platforms for root in soil, shoot, inflorescence, and seed. We demonstrated that high ambient temperature differentially impacts vegetative and reproductive organs through this pathway. Suppression of the PhyB-PIF4 components mimics the response to a high ambient temperature in wild-type plants. We also identified correlative responses to high ambient temperature between shoot and root tissues. This integrative and automated phenotyping was complemented by monitoring the changes in transcript levels in reproductive organs. Transcriptomic profiling of the pistils from plants grown under high ambient temperature identified key elements that may provide clues to the molecular mechanisms behind temperature-induced reduced fertilization rate, such as a downregulation of auxin metabolism, upregulation of genes involved auxin signalling, miRNA156 and miRN160 pathways, pollen tube attractants.
2024-07-03 | GSE263964 | GEO
Project description:Temperature is an important ecological condition, and sudden temperature changes in soil can induce stress in soil-dwelling invertebrates. Soil animals can move to more favorable habitats and/or adapt physiologically to a stressful environment. Hyperthermic conditions will impact gene expression as one of the first steps. We use a transcriptomics approach to identify the transcripts of which expression changed in response to heat stress in the springtail Folsomia candida using a 5,131 probe microarray. A temperature shift from 20°C to 30°C for 30 minutes significantly altered the expression of 142 genes, of which 116 were upregulated, and 26 downregulated. Many upregulated genes encoded heat shock proteins (Hsps) or enzymes involved in the synthesis of ATP, such as members of the electron transport chain. Furthermore, genes involved in oxidative stress and anion-transporting ATPases were upregulated. Downregulated were glycoside hydrolases, involved in catalysis of certain disaccharides, which indicate an accumulation of stress-protective disaccharides. The microarray results from this study, which were validated using quantitative RT PCR, reveal a mild response to heat shock in this soil invertebrate, relative to other organisms. This may be due to specific ecological factors during evolution of soil invertebrates, such as the relatively stable temperatures in the soil habitat. This study presents potential candidate genes for future functional studies concerning thermal stress in soil-dwelling invertebrates, like e.g., the investigation of the heat hardening process.
2010-01-16 | GSE17024 | GEO
Project description:To investigate the molecular basis of fluctuating temperature induced phenotypic plasticity, we ran genome-wide transcriptomic analysis on Drosophila melanogaster subjected to acclimation at constant (19 +/- 0 degree Celcius) and fluctuating (19 +/- 8 degree Celcius) temperatures and contrasted the induction of molecular mechanisms in adult males, adult females, and larvae. We investigated whether fluctuations act by permanently activating the involved mechanisms, or whether fluctuations repeatedly activate and repress mechanisms, during the hot (or heating) and the cold (or cooling) phase of thermal fluctuations. We show that adult flies acclimated to fluctuating temperatures tolerate high temperatures better than the constant temperature acclimated controls. Differential gene expression indicated that responses to thermal variability rely partly on life stage and sex specific mechanisms. Our results show that some of the involved mechanisms were permanently activated, while others tracked the thermal fluctuations. Further, for a number of genes, fluctuating temperature resulted in canalization of gene expression. Molecular mechanisms related to environmental sensing and chromatin reorganization seems to be important components of adaptive responses to thermal variability.
2022-04-04 | GSE150450 | GEO
Project description:During the application of thermal treatment, cell survival depends on biological factors (such as growth stage, culture conditions, and the composition of the stress medium) as well as physical factors inherent to the thermal process (including amplitude, duration at the stress temperature, and the rate of temperature increase). In this study, the yeast Saccharomyces cerevisiae BY4742 was exposed to two different thermal treatment kinetics in a nutrient medium after being cultivated in YPD medium at 25 °C for 48 hours (to reach the stationary growth phase). The thermal treatments were applied between 25 °C and 50 °C, followed by a 30-minute hold at 50 °C before returning to 25 °C. The yeast cells were exposed either to a heat shock (heating kinetics: 50 °C/min) or to a gradual temperature increase (heating kinetics: 0.5 °C/min). Generally, it is known that heat shock induces lower survival (30%) compared to the gradual increase (80%). In this study, we demonstrate that despite the small difference in survival rate (less than one logarithmic reduction in both cases), the transcriptomes vary significantly. The use of bioinformatics tools such as WGCNA revealed a correlation between differentially expressed gene clusters and phenotypic traits (measured by spectroscopy, fluorescence microscopy, flow cytometry, among others). Here, we focused on both mRNA and ncRNA (non coding RNA).
2024-09-04 | GSE276309 | GEO