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Transcriptome analysis of cultured human pulmonary artery smooth muscle cells (hPASMCs) to explore functional roles for CD26/DPP4 in stimulation with TGF-β1

ABSTRACT: Transcriptome analysis of cultured human pulmonary artery smooth muscle cells (hPASMCs) to explore functional roles for CD26/DPP4 in stimulation with TGF-β1

PROVIDER: PRJNA1046026 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26979073.fastq.gz	Fastqsanger.gz
	SRR26979074.fastq.gz	Fastqsanger.gz
	SRR26979075.fastq.gz	Fastqsanger.gz
	SRR26979076.fastq.gz	Fastqsanger.gz
	SRR26979077.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of cultured human pulmonary artery smooth muscle cells (hPASMCs) to explore functional roles for CD26/DPP4 in stimulation with TGF-β1

Project description:The purpose of this study was to evaluate the role of CD26/DPP4 in hPASMCs in response to TGF-β1. In experimental methods, hPASMCs were treated as follows. The hPASMCs were cultured in Smooth Muscle Cell Growth Medium 2 with 10% fetal bovine serum and were incubated at 37 ℃ in a 5% CO2 incubator. The cells at passage 6 were used for all experiments. The PASMCs were treated with CD26/DPP4 siRNA or non-specific control siRNA for 48 hours, then stimulated with TGF-β1 or PBS for 24 hours. Transcriptome analysis revealed that TGFβ treatment in cultured hPASMCs upregu-lated genes related to pulmonary vascular SMC proliferation, involving the Notch, PI3K-Akt, and NFκB signaling pathways.Conversely, DPP4-siRNA treatment in cultured hPASMCs downregulated these pathways.

2024-01-03 | GSE248794 | GEO

Transcriptome analysis of cultured human lung microvascular endothelial cells (HLMVECs) to explore functional roles for CD26/DPP4 in mediating inflammatory responses by LPS

Project description:Purpose: The purpose of this study is to evaluate the role of CD26/DPP4 in HLMVECs in response to the pro-inflammatory stimulus LPS. Methods: Four primary cell lines of HLMVECs were used. HLMVECs were cultured in endothelial growth medium-2 supplemented with 10% fetal bovine serum. The cells were incubated at 37°C in a 5% CO2 incubator and used at passages 6–8 for all experiments. 2 kinds of CD26/DPP4 siRNA were used. HLMVECS were treated with CD26/DPP4 siRNA or non-specific control siRNA or vehicle for 72 hours, then stimulated with LPS or PBS for 18 hours. Results: Varieties of DEGs were determined between groups leading to the enrichment analysis. Conclusions: The results suggest that CD26/DPP4 expression is related to multiple important functions in HLMVECs, including inflammation, barrier function, and regenerative processes.

2021-12-10 | GSE190405 | GEO

Transcriptome Analysis of Fibroblasts in Hypoxia-Induced Vascular Remodeling: Functional Roles of CD26/DPP4

Project description:In hypoxic pulmonary hypertension (PH), pulmonary vascular remodeling is characterized by the emergence of activated adventitial fibroblasts, leading to medial smooth muscle hyperplasia. Previous studies have suggested that CD26/dipeptidyl peptidase-4 (DPP4) plays a crucial role in the pathobiological processes in lung diseases. However, its role in pulmonary fibroblasts in hy-poxic PH remains unknown. Therefore, we aimed to clarify the mechanistic role of CD26/DPP4 in lung fibroblasts in hypoxic PH. Dpp4 knockout (Dpp4 KO) and wild-type (WT) mice were exposed to hypoxia for 4 weeks. The degree of PH severity and medial wall thickness was augmented in Dpp4 KO mice compared with that in WT mice, suggesting that CD26/DPP4 plays a suppressive role in the development of hypoxic PH. Transcriptome analysis of human lung fibroblasts cultured under hypoxic conditions revealed that TGFB2, TGFB3, and TGFA were all upregulated as differentially expressed genes after DPP4 knockdown with small interfering RNA treatment. These results suggest that CD26/DPP4 plays a suppressive role in TGFβ signal-regulated fibroblast ac-tivation under hypoxic conditions. Therefore, CD26/DPP4 may be a potential therapeutic target in patients with PH associated with chronic hypoxia.

2024-11-17 | GSE281533 | GEO

Transcriptome analysis of cultured human lung microvascular endothelial cells (HLMVECs) to explore functional roles for CD26/DPP4 in mediating inflammatory responses by LPS

Project description:Transcriptome analysis of cultured human lung microvascular endothelial cells (HLMVECs) to explore functional roles for CD26/DPP4 in mediating inflammatory responses by LPS

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Sheep dendritic cells CD26+ and CD26- versus total dendritic cells

Project description:Transcriptomic profiling of ovine skin lymph dendritic cell subsets CD26+ and CD26- compared to total enriched lymph dendritic cells. CD26+ and CD26- dendritic cells (DC) from sheep skin lymph were sorted by flow cytometry and were analysed for their transcriptomic profile. We demonstrate that the minor sheep CD26+ skin lymph DC subset shares significant transcriptomic similarities with mouse CD8a+ and human BDCA3+ DC. This allowed the identification of a common set of phenotypic characteristics for CD8a-like DC in the 3 mammalian species, i.e. SIRPlo, CADM1hi, CLEC9Ahi, DEC205hi, XCR1hi. Compared to CD26- DC, the sheep CD26+ DC show (1) potent stimulation of allogeneic naive CD8+ T cells with high selective induction of the Ifn-gamma and Il22 genes; (2) dominant efficacy in activating specific CD8+ T cells against exogenous soluble antigen; (3) selective expression of functional pathways associated to high capacity for antigen cross-presentation. Reference design (total enriched lymph dendritic cells) - Biological replicates: 2 sheep #10081 and #30066

2010-07-23 | E-GEOD-21889 | biostudies-arrayexpress

Transcriptome Analysis of Fibroblasts in Hypoxia-Induced Vascular Remodeling: Functional Roles of CD26/DPP4

Project description:Transcriptome Analysis of Fibroblasts in Hypoxia-Induced Vascular Remodeling: Functional Roles of CD26/DPP4

| PRJNA1184342 | ENA

Sheep dendritic cells CD26+ and CD26- versus total dendritic cells

2010-07-23 | GSE21889 | GEO

Mesothelioma cells: Control vs CD26 knockdown

Project description:CD26 is a prognostic factor in many cancers and implicated as therapeutic target. Knockdown of CD26 on mesothelioma cells H226 and JMN inhibit tumor progression. In order to elucidate CD26-mediated pathway for tumor progression, CD26 was knocked down on H226 and JMN cells, and RNAs from these cells were subjected to Microarray analysis.

2018-07-01 | GSE100241 | GEO

Genome-wide mRNA expression array to identify dysregulated genes between CD26+ and CD26- HT29 cells

Project description:Affymetrix HG-U133 Plus2 Array was applied to identify differentially expressed genes between FACS sorted CD26+ and CD26- subpopulations of HT29 colorectal cancer cell-line.

2022-01-14 | GSE192909 | GEO

Role of CD26 on oncogenic gene expression

Project description:Purpose: Investigate a mechanism of CD26 to promote tumor progression in mesothelioma. Background: CD26 play a role on tumor progression and is expressed in many kinds of human malignancy, including mesothelioma, renal cell carcinoma, and T cell leukemia/lymphoma. In mesothelioma, expression of CD26 is observed in mesothelioma cells, but not in non-neoplastic mesothelioma cells, suggesting a role of CD26 on tumor progression (Clin Cancer Res 13:4191-4200, 2007). CD26 is known to activate T-cells through associating with adenosine deaminase, mannose 6-phosphatic/insulin-like growth factor II receptor, or with CD45RO (Immunol Res 161:55-70, 1998). However, mechanism of CD26 to promote tumor progression was not known.

2014-12-01 | GSE52216 | GEO

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