Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1047286 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR27015238_1.fastq.gz | Fastqsanger.gz | |||
| SRR27015238_2.fastq.gz | Fastqsanger.gz | |||
| SRR27015239_1.fastq.gz | Fastqsanger.gz | |||
| SRR27015239_2.fastq.gz | Fastqsanger.gz | |||
| SRR27015240_1.fastq.gz | Fastqsanger.gz |
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Project description:Gene expression profiling in soybean under aluminum stress: mechanisms of magnesium amelioration of aluminum toxicity at gene expression level. Micro-molar concentrations of magnesium in culture solution has been shown to ameliorate Al toxicity in soybean and other leguminous species. Different theories have been proposed to explain the chemical mechanisms of how the two ions interact to neutralize the toxic effect of aluminum in the plant root system. To understand the molecular mechanisms of the phenomenon at the gene expression level in soybean, we undertook a comparative transcriptome analysis in Al-tolerant and Al-sensitive genotypes treated with aluminum alone or aluminum plus magnesium using DNA microarrays. The results revealed magnesium enhances Al-tolerance level in the Al-tolerant genotype by down-regulating genes commonly induced in response to Al toxicity. We hypothesized that the magnesium-mediated alleviation of Al toxicity in the Al-tolerant genotype emanates from reduction in energy expenditure of gene expression induced in response to Al stress. Conversely, magnesium appears to ameliorate Al toxicity in the sensitive genotype by dual mechanisms of increasing the expression level of several genes involved in Al-tolerance and decreasing the expression level of most genes. Keywords: Soybean, aluminum toxicity, magnesium, transcriptome Two genotypes: PI 416937 (p) and Young (y); two treatments: Aluminum (Al) or Al+magnesium (Mg); two time points: 12 and 72 hrs; 3 replicates.
2010-10-04 | E-GEOD-18518 | biostudies-arrayexpress
Project description:Gene expression profiling in soybean under aluminum stress: mechanisms of magnesium amelioration of aluminum toxicity at gene expression level. Micro-molar concentrations of magnesium in culture solution has been shown to ameliorate Al toxicity in soybean and other leguminous species. Different theories have been proposed to explain the chemical mechanisms of how the two ions interact to neutralize the toxic effect of aluminum in the plant root system. To understand the molecular mechanisms of the phenomenon at the gene expression level in soybean, we undertook a comparative transcriptome analysis in Al-tolerant and Al-sensitive genotypes treated with aluminum alone or aluminum plus magnesium using DNA microarrays. The results revealed magnesium enhances Al-tolerance level in the Al-tolerant genotype by down-regulating genes commonly induced in response to Al toxicity. We hypothesized that the magnesium-mediated alleviation of Al toxicity in the Al-tolerant genotype emanates from reduction in energy expenditure of gene expression induced in response to Al stress. Conversely, magnesium appears to ameliorate Al toxicity in the sensitive genotype by dual mechanisms of increasing the expression level of several genes involved in Al-tolerance and decreasing the expression level of most genes. Keywords: Soybean, aluminum toxicity, magnesium, transcriptome
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Project description:Aluminum (Al) toxicity in plants is one of the primary constraints in crop production. Al³⁺, the most toxic form of Al, is released into soil under acidic conditions and causes extensive damage to plants, especially in the roots. In rice, Al tolerance requires the ASR5 gene, but the molecular function of ASR5 has remained unknown. This data establish a comparative study of miRNAome profiles in ASR5 knockdown rice plants (ssp. Japonica cv. Nipponbare) under Al stress conditions.
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Project description:Aluminum (Al)–tolerant phosphobacteria can improve plant performance in acidic soils by increasing Al complexing and phosphorus (P) availability. However, it is almost unknown how Al stress along with P deficiency affect the bacterial biochemistry and physiology. Because high Al levels and low P availability often occur simultaneously in acidic soils, we have evaluated the single and mutual effects of a high Al stress and P deficiency on the proteome of the Al‒tolerant phosphobacteria strain Enterobacter sp. RJAL6. This strain was previously isolated from the rhizosphere of Lollium perenne plants grown in acidic soil. The strain was cultivated in mineral media modified to contain i) high P (1.4 mM) in the absence of Al, ii) high P (1.4 mM) and high Al (10 mM), iii) low P (0.05 mM) in the absence of Al, and iv) low P (0.05 mM) and high Al (10 mM). Total proteins from bacterial cells were extracted at the end of the exponential phase of growth and subjected to high–throughput proteomics analysis. The results showed that P deficiency was mainly associated with an upregulation of the P metabolism proteins subject to Pho regulon control, including phosphatases and transporters involved in the uptake of organophosphorus compounds such as phosphomonoesters, phosphonates and glycerol–3–phosphate. Aluminum exposure primarily decreased the expression of iron (Fe)–sulfur and haem-containing proteins with a concomitant upregulation of Fe acquisition and metabolism proteins, including siderophore precursors and receptors of Fe–chelator complexes. Here, we demonstrated the preponderant role that Al plays in the adjustment of Fe homeostasis, and consequently in the central metabolism of the bacteria. This is the first report of a proteomic study of the interaction between high Al and P deficiency in acidic soil–adapted bacteria. This knowledge is crucial for developing bioinoculants for crops affected by both Al toxicity and P deficiency.
2023-09-27 | PXD041868 | Pride
Project description:Aluminum (Al)–tolerant phosphobacteria can improve plant performance in acidic soils by increasing Al complexing and phosphorus (P) availability. However, it is almost unknown how Al stress along with P deficiency affect the bacterial biochemistry and physiology. Because high Al levels and low P availability often occur simultaneously in acidic soils, we have evaluated the single and mutual effects of a high Al stress and P deficiency on the proteome of the Al‒tolerant phosphobacteria strain Enterobacter sp. 198. This strain was previously isolated from the rhizosphere of Lollium perenne plants grown in acidic soil. The strain was cultivated in mineral media modified to contain i) high P (1.4 mM) in the absence of Al, ii) high P (1.4 mM) and high Al (10 mM), iii) low P (0.05 mM) in the absence of Al, and iv) low P (0.05 mM) and high Al (10 mM). Total proteins from bacterial cells were extracted at the end of the exponential phase of growth and subjected to high–throughput proteomics analysis. The results showed that P deficiency was mainly associated with an upregulation of the P metabolism proteins subject to Pho regulon control, including phosphatases and transporters involved in the uptake of organophosphorus compounds such as phosphomonoesters, phosphonates and glycerol–3–phosphate. Aluminum exposure primarily decreased the expression of iron (Fe)–sulfur and haem-containing proteins with a concomitant upregulation of Fe acquisition and metabolism proteins, including siderophore precursors and receptors of Fe–chelator complexes. Here, we demonstrated the preponderant role that Al plays in the adjustment of Fe homeostasis, and consequently in the central metabolism of the bacteria. This is the first report of a proteomic study of the interaction between high Al and P deficiency in acidic soil–adapted bacteria. This knowledge is crucial for developing bioinoculants for crops affected by both Al toxicity and P deficiency.
2023-09-27 | PXD041866 | Pride
Project description:Aluminum (Al)–tolerant phosphobacteria can improve plant performance in acidic soils by increasing Al complexing and phosphorus (P) availability. However, it is almost unknown how Al stress along with P deficiency affect the bacterial biochemistry and physiology. Because high Al levels and low P availability often occur simultaneously in acidic soils, we have evaluated the single and mutual effects of a high Al stress and P deficiency on the proteome of the Al‒tolerant phosphobacteria strain Klebsiella sp. RCJ4. This strain was previously isolated from the rhizosphere of Lollium perenne plants grown in acidic soil. The strain was cultivated in mineral media modified to contain i) high P (1.4 mM) in the absence of Al, ii) high P (1.4 mM) and high Al (10 mM), iii) low P (0.05 mM) in the absence of Al, and iv) low P (0.05 mM) and high Al (10 mM). Total proteins from bacterial cells were extracted at the end of the exponential phase of growth and subjected to high–throughput proteomics analysis. The results showed that P deficiency was mainly associated with an upregulation of the P metabolism proteins subject to Pho regulon control, including phosphatases and transporters involved in the uptake of organophosphorus compounds such as phosphomonoesters, phosphonates and glycerol–3–phosphate. Aluminum exposure primarily decreased the expression of iron (Fe)–sulfur and haem-containing proteins with a concomitant upregulation of Fe acquisition and metabolism proteins, including siderophore precursors and receptors of Fe–chelator complexes. Here, we demonstrated the preponderant role that Al plays in the adjustment of Fe homeostasis, and consequently in the central metabolism of the bacteria. This is the first report of a proteomic study of the interaction between high Al and P deficiency in acidic soil–adapted bacteria. This knowledge is crucial for developing bioinoculants for crops affected by both Al toxicity and P deficiency.
2023-09-27 | PXD041867 | Pride