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ABSTRACT: RNA sequencing following KLF12 knockdown in KYSE30 cells

PROVIDER: PRJNA1047887 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27027183_1.fastq.gz	Fastqsanger.gz
	SRR27027183_2.fastq.gz	Fastqsanger.gz
	SRR27027184_1.fastq.gz	Fastqsanger.gz
	SRR27027184_2.fastq.gz	Fastqsanger.gz
	SRR27027185_1.fastq.gz	Fastqsanger.gz

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Homo sapiens

Project description:RNA-seq in KYSE30 and KYSE150 cells following KLF12 overexpression

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Project description:RNA sequencing following TRIM27 knockdown in KYSE30 cells

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Mass spectrometry data following immunoprecipitation of Flag-KLF12

Project description:Mass spectrometry data following immunoprecipitation of KLF12 in KYSE30 and HEK293 cell lines, aimed at discovering proteins that interact with KLF12.

2023-12-19 | PXD047917 |

RNA sequencing analysis of OTUD6B-knockdown KYSE30 and KYSE450 cells

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are using RNA sequencing analysis to test the effects of OTUD6B-knockdown on the mRNA of KYSE30 and KYSE450 cells. Methods: KYSE30 and KYSE450 cells were transfected with negative control or siOTUD6B for 48 hours in 1640 medium plus 10% serum. Three independent replicates were plated, transfected in parallel for each negative control and siOTUD6B. Results: Log-fold changes of mRNAs between negative control and siOTUD6B group were selected with a significance threshod of p<0.05. Conclusions: Our study determined the mRNA changes of OTUD6B-knockdown KYSE30 and KYSE450 cells.

2023-04-01 | GSE209521 | GEO

Transcriptomes Analysis of LHX2-knockdown in KYSE30 and KYSE510 cells

Project description:Purpose: To fully realize the potential molecular mechanism that LHX2 promotes ESCC progression Methods: Total RNA of LHX2-knockdown KYSE30/KYSE510 and control cells was extracted with TRIzol Reagent. RNA libraries were constructed using an Illumina TruSeq RNA Sample Preparation kit according to the manufacturer’s protocol. A total of 150 base paired-end reads were sequenced using the Novaseq 6000 S4 platform. Results: We identified 26008 transcripts in KYSE30 control and KYSE30 LHX2-knockdown cells ,and 25561 transcripts in KYSE510 control and KYSE510 LHX2-knockdown cells. Conclusions: Our study represents the analysis of LHX2-knockdown ESCC cells, generated by RNA-seq.

2023-04-07 | GSE209942 | GEO

RNA sequencing analysis of OTUD6B-knockdown KYSE30 and KYSE450 cells

Project description:RNA sequencing analysis of OTUD6B-knockdown KYSE30 and KYSE450 cells

| PRJNA861139 | ENA

Differentially expressed genes between ACh-stimulated KYSE30 cells and the respective control cells

Project description:Purpose:RNA sequencing explored the possible altered gene transcriptome and signal pathways between ACh-stimulated KYSE30 cells and the respective control cells. Methods:RNA sequencing explored the ACh-stimulated KYSE30 cells and the respective control cells.

2022-05-11 | GSE202482 | GEO

Project description:ChIP-seq Analysis of KLF12 in KYSE30 Cells with and without TRIM27 Overexpression

| PRJNA1049742 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Klf12-/- Lymphocyte Transcriptomes

Project description:The goal of this study is to determine the role of the transcription factor, Klf12, in mouse NK cells. Transcriptome profiling by RNA-Seq of wild-type and Klf12-deficient mouse CD4+ T cells, CD8+ T cells, B cells, and NK cells. We found that Klf12-deficient mouse NK cells express more transcripts of Btg3 and have a proliferative impairment compared to wild-type mouse NK cells in a competitive setting.

2020-04-13 | GSE128962 | GEO

Next generation sequencing facilitates transcriptome analyses of mock and RHCG transfected esophageal squamous cell carcinoma cell line KYSE30 cells

Project description:We obtained transcriptome profiling (RNA-seq) of human esophageal squamous cell carcinoma cell line KYSE30 cells stabley transfected with pIRES2-EGFP vector or human RHCG-expression vector by using next generation sequencing.

2019-09-26 | GSE87664 | GEO

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