Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1048629 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Differentially expressed genes between osteochondroprogenitor sub-populations
| PRJNA270937 | ENA
Project description:A quantitative proteomics assay was used to analyze the protein profile and screen differentially expressed proteins (DEPs) between the GRK4 overexpression with lentivirus vector(OE) and normal control lentivirus vector (NC) HepG2 cells.
2023-02-08 | PXD039951 |
Project description:In the current study, to figure out the regulation pattern of TfR1, we knocked down TFRC expression level by shRNA in HeLa cells. RNA-sequencing (RNA-seq) was used to analyze the global transcript level and alternative splicing (AS) on knockdown-treated (KD) and normal control (NC) cell samples. 629 differentially expressed genes (DEGs) were identified between OE and NC, and Gene ontology (GO) and KEGG analysis for DEGs were carried out. It was found that multiple DEGs were involved in O-glycan processing, protein modification, response to hypoxia and ATP catabolic process, indicating the down-regulated expression of TfR1 extensively disturbed cell physiology.
2022-05-08 | GSE131598 | GEO
Project description:We aim, among other things, to characterize olfactory receptor (OR) genes wich are differentially expressed between olfactory epithelium (OE) and other organ tissues, in humans. Six OE samples and ten non-OE samples, including two samples of the following tissues: liver, kidney, lung, testis, and heart.
2010-06-11 | E-GEOD-5969 | biostudies-arrayexpress
Project description:The transferrin receptor 1 (TfR1), encoded by TFRC gene, is the gatekeeper of cellular iron uptake for cells. A variety of molecular mechanisms are at work to tightly regulate TfR1 expression, and abnormal TfR1 expression was associated with diseases. In the current study, to figure out the regulation pattern of TfR1, we cloned and overexpressed human TFRC gene in HeLa cells. RNA-sequencing (RNA-seq) was used to analyze the global transcript level on overexpression-treated (OE) and normal control (NC) cell samples. 1669 differentially expressed genes (DEGs) were identified between OE and NC, and Gene ontology (GO) analysis for DEGs were carried out. It was found that lots of DEGs were associated with ion transmembrane transport and immunity. Moreover, the network was constructed on basis of DEGs regulating ion transport and immunity, the results revealed that TFRC was the node gene of the network, further suggesting that precisely controlled TfR1 expression might be not only essential for iron homeostasis, but also globally important for cell physiology, including ion transport and immunity.
2021-08-27 | GSE130048 | GEO
Project description:Differentially expressed genes between pathological and normal palmar aponeurosis
| PRJNA778866 | ENA
Project description:Purpose: The clinical significance, biological function of AC093797.1 are still unexplored in HCC or any other malignant tumor. In this study, we aimed to investigate the biological function of AC093797.1 in HCC and screen the candidate hub genes and pathways related to hepatocarcinogenesis via RNA-sequencing. Methods: The effects of AC093797.1 on tumor growth in vivo was clarified by nude mice tumor formation experiments. The 5-weeks old female BALB/c nude mice weighing 15-20g were maintained under specific-pathogen-free conditions and randomly divided into OE-AC093797 group and negative control (NC) group (n=5 per group). Then the HCCLM3 cells stably transfected with control vector pcDNA3.1 or the overexpression vector OE-AC093797.1 (5×10^7cells in 100μl) were subcutaneously injected into nude mice. Calculate the volume of the tumors by the following formula: Vtumor= length × width2×π/6. When the tumor grows to about 2000 mm3, the mice were sacrificed after anesthesia and their tumor tissues were isolate. Three mice of each group were used to RNA-sequencing and bioinformatics analysis based on subcutaneous tumor tissue. Results: The “DESeq2” package of R software was used to filtered the differential expressed genes between the OE-AC093797 group and the NC group. The cut-off criteria of differential expressed genes was |log2FoldChange|>1.5 and adj. p value (FDR)<0.05. 710 differentially expressed genes (243 up-regulated genes and 467 down-regulated genes) were screened between groups, which mainly enriched in amino acid metabolism，extracellular matrix structure constituent, cell adhesion molecules cams，signaling to Ras, and signaling to ERKs, etc. via gene ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and gene set enrichment analysis (GSEA). Conclusion: AC093797.1 can inhibits tumor group might via reprogram cell metabolism or Extracellular matrix dynamics in HCC.
2021-11-19 | GSE186933 | GEO
Project description:The goals of this study are to compare the differentially expressed genes between NC and shMETTL14 groups
2022-07-20 | GSE173520 | GEO
Project description:We report the impact of altered CMPK2 expression in THP1 macrophages. CMPK2 silencing THP1 macrophages (CM) were constructed by stably expressing siRNA against CMPK2. Scrambled siRNA expressing THP1 cells were used as control (SC). THP1 macrophages stably expressing CMPK2 fused with mCherry was used as CMPK2 overexpressing macrophages (OE). Control cells were constructed by overexpressing mCherry alone (VC). The RNA sequencing was used to identify genes that are differentially expressed in CMPK2 modified THP1 macrophages.
2022-11-11 | GSE199951 | GEO
Project description:We aim, among other things, to characterize olfactory receptor (OR) genes wich are differentially expressed between olfactory epithelium (OE) and other organ tissues, in chimpanzees (Pan troglodytes), and to make comparisons with human data from a previous series using the same platform. Keywords: Comparative Four OE samples and eight non-OE samples, including two samples of the following tissues: heart, liver, lung, and testis.
2008-10-01 | E-GEOD-11156 | biostudies-arrayexpress