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Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation [RNA-seq]

ABSTRACT: Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation [RNA-seq]

PROVIDER: PRJNA1049049 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27099200.fastq.gz	Fastqsanger.gz
	SRR27099201.fastq.gz	Fastqsanger.gz
	SRR27099202.fastq.gz	Fastqsanger.gz
	SRR27099203.fastq.gz	Fastqsanger.gz
	SRR27099204.fastq.gz	Fastqsanger.gz

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Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation

Project description:Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation

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Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation [Ribo-seq]

Project description:Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation [Ribo-seq]

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Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation

Project description:The transcriptional control of sporulation in Bacillus subtilis is reasonably well understood, but its translational control is underexplored. Here, we use RNA-seq, ribosome profiling and fluorescence microscopy to study the translational dynamics of B. subtilis sporulation. We identify two events of translation silencing and describe spatiotemporal changes in subcellular localization of ribosomes during sporulation. We investigate the potential regulatory role of ribosomes during sporulation using a strain lacking zinc-independent paralogs of three zinc-dependent ribosomal proteins (L31, L33 and S14). The mutant strain exhibits delayed sporulation, reduced germination efficiency, dysregulated translation of metabolic and sporulation-related genes, and disruptions in translation silencing, particularly in late sporulation. This SuperSeries is composed of the SubSeries listed below.

2023-12-06 | GSE249450 | GEO

Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation [Ribo-seq]

Project description:Translational control during the intricate process of sporulation in Bacillus subtilis as a response to nutrient limitation is still underexplored. Here, we employed a comprehensive approach including RNA-seq, ribosome profiling and fluorescence microscopy to dissect the translational landscape of B. subtilis during sporulation. We identified two events of translation silencing and described the spatiotemporal changes in the subcellular location of translational machinery during sporulation. Using a triple knock-out strain (3KO) of zinc-independents paralogs of three zinc-dependent ribosomal proteins L31, L33 and S14, we investigated the potential regulatory role of ribosome during sporulation. The 3KO strain exhibited delayed sporulation, reduced germination efficiency, and dysregulated translation including expression of key metabolic and sporulation-related genes as well as disruptions in translation silencing, particularly in late sporulation.

2023-12-06 | GSE249448 | GEO

Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation [RNA-seq]

2023-12-06 | GSE249449 | GEO

Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation

Project description: Not available

| S-EPMC11339384 | biostudies-literature

DIURNAL RHYTHMS SPATIALLY AND TEMPORALLY ORGANIZE AUTOPHAGY

Project description:RAW spectrum files associated with "DIURNAL RHYTHMS SPATIALLY AND TEMPORALLY ORGANIZE AUTOPHAGY"

2019-01-15 | MSV000083314 | MassIVE

Elongation Factor P is important for sporulation initiation

Project description:The universally conserved protein Elongation Factor P facilitates translation at amino acids that form peptide bonds with low efficiency, particularly poly-proline tracts. Despite its wide conservation, it is not essential in most bacteria and its physiological role remains unclear. Here, we show that EF-P affects the process of sporulation initiation in the bacterium Bacillus subtilis. We observe that lack of EF-P delays expression of sporulation-specific genes. Using ribosome profiling, we observe that expression of spo0A, encoding a transcription factor that functions as the master regulator of sporulation, is lower in a ∆efp strain as compared to the wildtype. Ectopic expression of Spo0A rescues the sporulation initiation phenotype, indicating that reduced spo0A expression explains the sporulation defect in ∆efp cells. Since Spo0A is the earliest sporulation transcription factor, these data suggest that sporulation initiation can be delayed when protein synthesis is impaired.

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Project description:Mapping alterations spatially and temporally during early stages of breast tumourigenesis

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Temporally & Spatially-Resolved Microproteomics and MALDI MSI Lipidomic Analysis for Traumatic Brain Injury

Project description:Using an experimental TBI rat model of mild/moderate Controlled Cortical Impact (CCI) injury, we combined large-scale proteomics identification and relative quantification using Spatially-Resolved Microproteomics with MALDI MS Imaging of Lipids. Spatially by studying different regions in the brain post injury in a coronal view, with main focus on the injury site itself. Temporally by studying the acute and subacute phase post injury, including injured rat brains at 1 day, 3 days, 7 days, and 10 days post injury. Direct on-tissue micro-digestion followed by micoextraction from 1 mm2 surface area within the injured cortical tissue were subjected to LC-MS & MS/MS analysis using HR MS. In addition, several identified potential biomarkers within our study were used to stimulate dorsal root ganglion (DRG), astrocyte, and macrophage cell lines to obtain a better understanding of their role and contribution in the injury.

2019-06-19 | PXD011262 | Pride

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