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Gene expression profile at single cell level of stromal cells(SCs) from the periosteum and bone marrow

ABSTRACT: Gene expression profile at single cell level of stromal cells(SCs) from the periosteum and bone marrow

PROVIDER: PRJNA1049438 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27117524_1.fastq.gz	Fastqsanger.gz
	SRR27117524_2.fastq.gz	Fastqsanger.gz
	SRR27117525_1.fastq.gz	Fastqsanger.gz
	SRR27117525_2.fastq.gz	Fastqsanger.gz
	SRR27117526_1.fastq.gz	Fastqsanger.gz

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Gene expression profile at single cell level of stromal cells(SCs) from the periosteum and bone marrow

Project description:Stromal cells in bone are considered as a heterogenous population that have different locations and functions, though all of them can self-renew and differentiate into osteoblasts. We used single cell RNA sequencing(scRNA-seq) to dissect the heterogeneity of Stromal cell in the bone.

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Periosteum contains skeletal stem cells with high bone regenerative potential controlled by Periostin

Project description:Bone regeneration relies on the activation of skeletal stem cells (SSCs) that still remain poorly characterized. Here, we show that periosteum contains SSCs with high bone regenerative potential compared to bone marrow stromal cells/skeletal stem cells (BMSCs) in mice. Although periosteal cells (PCs) and BMSCs are derived from a common embryonic mesenchymal lineage, post-natally PCs exhibit greater clonogenicity, growth and differentiation capacity than BMSCs. During bone repair, PCs can efficiently contribute to cartilage and bone, and integrate long-term after transplantation. Molecular profiling uncovers genes encoding Periostin and other extracellular matrix molecules associated with the enhanced response to injury of PCs. Periostin gene deletion impairs PC functions and fracture consolidation. Periostin-deficient periosteum cannot reconstitute a pool of PCs after injury demonstrating the presence of SSCs within periosteum and the requirement of Periostin in maintaining this pool. Overall our results highlight the importance of analyzing periosteum and PCs to understand bone phenotypes.

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