Project description:Ruminant livestock are one of the major contributors to carbon emission contributing the global warming issue. Methane (CH4) produced from enteric microbial fermentation of feed in the reticulo-rumen are known to differ between sheep with different digestive function and fermentation products such as metabolites. However, the molecular mechanism underpinning differences in methane emission remains to be fully elucidated. We extracted a membrane and cytosolic protein fraction of rumen epithelium proteins from both high (H) and low (L) CH4 emitting sheep. Protein abundance differences between the phenotypes were quantified using SWATH-mass spectrometry. We identified 92 proteins annotated as cell surface transporters, of which only solute carrier family (SLC) 40A1 had a greater fold change of protein expression in the high methane emission phenotype. The main difference in protein abundance we found were related to the metabolism of glucose, lactate and processes of cell defence against microbes in the epithelium of sheep in each group. To best of our knowledge, this represents one of the most comprehensive proteomes of ovine rumen epithelium to date.
Project description:a shotgun approach and bioinformatics analyses to investigate and compare proteomic profile of sheep rumen epithelium tissue on day 0, 15, 30, 45 and 60 of age.
2023-03-11 | PXD034787 | Pride
Project description:Archaeal community composition of rumen content of Hu sheep
Project description:In order to investigate the diurnal oscillations of ruminal bacteria, and their responses to the changes in different feeding patterns, we conducted an animal experiment by feeding the sheep ad libitum with a hay-based diet (50% of alfalfa hay and 46% of oats hay) and a grain-based diet (45% of corn meal and 11% of soybean meal) for 30 days, and ruminal fluid samples were collected at six different timepoints from T2 to T22 in one day, and the composition and diversity of the bacterial communities in rumen microbiomes of the sheep in the Grain-diet and Hay-diet groups at different timepoints were analyzed through 16S rRNA sequencing.
2025-01-08 | GSE286149 | GEO
Project description:Rumen microbiome of Hu sheep
| PRJNA1180905 | ENA
Project description:Rumen microbiome of Hu sheep
| PRJNA1180906 | ENA
Project description:Microorganisms in Hu sheep rumen
| PRJNA1051932 | ENA
Project description:Hu sheep, East Friensian sheep, and East Friensian*Hu Crossbreed sheep rumen microbiome