Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1050323 | ENA |
REPOSITORIES: ENA
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Project description:This research work investigates the expression of the genes involved in flavor compound production in two hybrids between Saccharomyces cerevisiae and S. kudriavzevii under low (12°C) and moderate fermentation temperatures (28°C).
2015-09-05 | GSE30779 | GEO
Project description:This research work investigates the expression of the genes involved in flavor compound production in three different Saccharomyces species (S. cerevisiae, S. bayanus var. uvarum and S. kudriavzevii) under low (12°C) and moderate fermentation temperatures (28°C).
2014-06-23 | GSE30778 | GEO
Project description:Different intensities of high temperatures affect the growth of photosynthetic cells in nature. To elucidate the underlying mechanisms, we cultivated the unicellular green alga Chlamydomonas reinhardtii under highly controlled photobioreactor conditions and revealed systems-wide shared and unique responses to 24-hour moderate (35oC) and acute (40oC) high temperatures and subsequent recovery at 25oC. We identified transcripts/proteins with unique differential regulation at 35oC, uncovering previously overlooked novel elements in response to moderate high temperature. Heat at 35oC increased transcripts/proteins involved in gluconeogensis/glyoxylate-cycle for carbon uptake, promoted growth, and increased starch accumulation. Heat at 40oC inhibited growth, resulting in carbon uptake over usage and increased starch accumulation. Heat at 35oC transiently arrested the cell cycle followed by partial synchronization while 40oC inhibited DNA replication and arrested the cell cycle. Both high temperatures induced photoprotection, while 40oC decreased photosynthetic efficiencies, distorted thylakoid/pyrenoid ultrastructure, and affected the carbon concentrating mechanism. We demonstrated increased transcript/protein correlation during heat, which decreased during recovery, suggesting reduced post-transcriptional regulation during heat may help coordinate heat tolerance activities efficiently. During recovery after both treatments, transcripts/proteins related to DNA synthesis increased while those involved in photosynthetic light reactions decreased. We propose down-regulating photosynthetic light reactions during DNA replication benefits cell cycle resumption by reducing ROS production. Our results provide potential targets to increase thermotolerance in algae and crops.
2022-01-01 | GSE182207 | GEO
Project description:Different intensities of high temperatures affect plant growth and yield in the field, but the underlying mechanisms remain elusive. Using the unicellular green alga, Chlamydomonas reinhardtii, under highly controlled photobioreactor conditions, we revealed systems-wide shared and unique responses to 24-hour moderate (35oC) and acute (40oC) high temperatures and subsequent recovery at 25oC. Heat at 35oC increased transcripts/proteins involved in gluconeogensis/glyoxylate-cycle for carbon uptake, promoted growth, and increased starch accumulation. Heat at 40oC inhibited growth, resulting in carbon uptake over usage and increased starch accumulation. Heat at 35oC transiently inhabited the cell cycle followed by partial synchronization while 40oC inhibited DNA replication and arrested cell cycle. Both high temperatures induced photoprotection, while 40oC decreased photosynthetic efficiencies, distorted thylakoid/pyrenoid ultrastructure, and affected carbon concentrating mechanism. We demonstrated increased transcript/protein correlation during heat, which decreased during recovery, suggesting reduced post-transcriptional regulation during heat may help coordinate heat tolerance activities efficiently. During recovery after both treatments, transcripts/proteins related to DNA synthesis increased while those involved in photosynthetic light reactions decreased. We propose down-regulating photosynthetic light reactions during DNA replication benefits cell cycle resumption by reducing ROS production. Furthermore, we identified transcripts/proteins with unique differential regulation at 35oC, uncovering previously overlooked novel elements in response to moderate high temperature. Our research improves understanding of heat responses in photosynthetic cells and provides potential targets to increase thermotolerance in algae and crops.
2022-06-09 | PXD027778 | Pride
Project description:The archaea Cuniculiplasma divulgatum are abundant in acidic environments with low to moderate temperatures. However, the molecular mechanisms underlying its ability to thrive at low temperatures remain unexplored. Using mass spectrometry (MS)-based proteomics, we analysed the effect of short-term (3 h) exposure to cold shock. The C. divulgatum genome encodes 2,016 protein-coding genes, from which 819 proteins were identified in the cells grown under optimal conditions. In line with the peptidolytic lifestyle of C. divulgatum, its intracellular proteome revealed the abundance of proteases, ABC transporters and cytochrome C oxidase. From 747 quantifiable polypeptides, the levels of 582 proteins showed no change after the cold shock (the core proteome), whereas 104 proteins were upregulated suggesting that they might be contributing to cold adaptation. The highest increase in protein levels was found for the metal-dependent hydrolase, FAD-dependent oxidoreductase, aspartate carbamoyltransferase regulatory chain proteins, 2-oxoacid ferredoxin oxidoreductase and ATPase-V type ATP synthase. Furthermore, the cold shock induced a substantial increase (3.5 % and 9.0 %) in the levels of two most abundant intracellular proteins, chaperonin and glutamate dehydrogenase. This study has outlined potential mechanisms of environmental fitness of Cuniculiplasma spp. allowing them to colonise and survive in acidic settings at low/ moderate temperatures.
2020-05-26 | PXD017828 | Pride
Project description:Diversity of culturable thermophiles
| PRJEB22074 | ENA
Project description:Recent studies have revealed that at lower cultivation temperatures (25°C) much higher percentage of correctly folded recombinant proteins can be extracted from inclusion bodies. The goal of our research was to investigate mechanisms determining characteristics of non-classical inclusion bodies production using gene expression profiling. Two strains of recombinant E. coli [BL21 (DE3)] grown at three different temperatures (25°C, 37°C and 42°C) were included in experiment. Gene expression was studied in two recombinant strains (production and control strain), grown at three different temperatures (25, 37 and 42 oC) in three biological replicates. Cells were harvesed at OD=4, except for the cells grown at 25 oC, here they were harvested at OD=4 and OD=10.
2010-11-24 | E-GEOD-25561 | biostudies-arrayexpress
Project description:Regulatory role of PIF4 in C24xCol Arabidopsis hybrid and moderate and high ambient temperatures
| PRJNA660450 | ENA