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Transcriptome and translatome changes upon restored YB-1 or YB-3 expression in double knockout HEK293T ΔYB-1ΔYB-3 cells

ABSTRACT: Transcriptome and translatome changes upon restored YB-1 or YB-3 expression in double knockout HEK293T ΔYB-1ΔYB-3 cells

PROVIDER: PRJNA1051095 | ENA |

REPOSITORIES: ENA

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Changes in transcriptome and translatome upon YB-1 or YB-3 expression in double knockout HEK293T ΔYB-1ΔYB-3 cells

Project description:In this study, we generated transcriptome profiles from HEK293T, HEK293T ΔYB-1ΔYB-3, and HEK293T ΔYB-1ΔYB-3 with the restored expression of YB-1 and/or YB-3. The data are used to explore YB-1 and YB-3 influence on transcriptome and translatome and their functional interchangeability in transcription and translation.

2024-01-18 | GSE249895 | GEO

Transcriptome (RNA-Seq), translatome (Ribo-Seq), and YB-1/YB-3-bound mRNAs (RIP-Seq) of HEK293T, HEK293TdeltaYB-1, and HEK293T+HA-YB-1 cells

Project description:We present results of RNA-Seq, Ribo-Seq, and RIP-Seq (YB-1, YB-3) experiments performed in HEK293T cells, as well as in HEK293T cells with YB-1 knockout and overexpression. The data shows YB-1 function as a global translation inhibitor and YB-3 ability to substitute YB-1 in its function in YB-1 knockout mutant.

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Changes in transcriptome and translatome upon YB-1 or YB-3 expression in double knockout HEK293T ΔYB-1ΔYB-3 cells

Project description:Changes in transcriptome and translatome upon YB-1 or YB-3 expression in double knockout HEK293T ΔYB-1ΔYB-3 cells

| PRJNA1051101 | ENA

Transcriptome (RNA-Seq), translatome (Ribo-Seq), and YB-1/YB-3-bound mRNAs (RIP-Seq) of HEK293T, HEK293TdeltaYB-1, and HEK293T+HA-YB-1 cells

Project description:Transcriptome (RNA-Seq), translatome (Ribo-Seq), and YB-1/YB-3-bound mRNAs (RIP-Seq) of HEK293T, HEK293TdeltaYB-1, and HEK293T+HA-YB-1 cells

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PAR-CLIP analysis of YB-1 RNA binding in HEK293T cells

Project description:In this study, we performed PAR-CLIP experiment in HEK293T to explore RNAs directly bound by YB-1.

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Transcriptome and translatome changes upon restored YB-1 or YB-3 expression in double knockout HEK293T ΔYB-1ΔYB-3 cells

Project description:This SuperSeries is composed of the SubSeries listed below.

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Translatome analyses of HEK293T upon NSP12 overexpression

Project description:Translatome analyses of HEK293T upon NSP12 overexpression

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PAR-CLIP analysis of YB-1 RNA binding in HEK293T cells

Project description:PAR-CLIP analysis of YB-1 RNA binding in HEK293T cells

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HEK293T cell translatome analysis upon 6-MP treatment. Related to Supplementary Table 6 from An et al (2020)

Project description:HEK293T cells were treated with 6-MP and harvested at various time point (6, 9 and 18h). Translatome was measure by Azidohomoalanine (AHA) incorporation, Biotin-click, and streptavidin enrichment.

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Class I HDAC inhibitors enhance YB-1 acetylation and oxidative stress to block sarcoma metastasis

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