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CUT&TAG analysis of H3K27ac in granulosa cells from control and POI patients

ABSTRACT: CUT&TAG analysis of H3K27ac in granulosa cells from control and POI patients

PROVIDER: PRJNA1052695 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27212587_1.fastq.gz	Fastqsanger.gz
	SRR27212587_2.fastq.gz	Fastqsanger.gz
	SRR27212588_1.fastq.gz	Fastqsanger.gz
	SRR27212588_2.fastq.gz	Fastqsanger.gz
	SRR27212589_1.fastq.gz	Fastqsanger.gz

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CUT&TAG analysis of H3K27ac in granulosa cells from control and POI patients

Project description:To reveal the role of NCOA7 in cellular senescence, we performed the CUT&TAG assay using the H3K27ac antibody to map acetylation in granulosa cells from control and POI patients. We conducted the DNA sequencing of libraries from CUT&TAG assay using the H3K27ac antibody in human primary granulosa cells.

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H3K27ac CUT&Tag iEC reprogramming

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Project description:In this study, we found that H3K23ac, H3K27ac, and H4K5ac level are elevated in VSMCs of aneurysmal aortas. To further explore the potential functional significance of H3K23ac, H3K27ac, and H4K5ac in aortic aneurysm, we performed genome-wide cleavage under targets and tagmentation (CUT&Tag) analysis to identify candidate genes regulated by H3K23ac, H3K27ac, or H4K5ac in rat VSMC. Following CUT&Tag, H3K23ac, H3K27ac, or H4K5ac-associated DNAs were amplified using non-biased conditions, labeled, and sequenced with Illumina NovaSeq 150PE.

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Project description:Purpose: To transcriptome widely reveal histone acetylation modification H3K9ac and H3K27ac regulation by Rbm25, we performed Cleavage Under Targets and Tagmentation (CUT&Tag) analysis of wild-type (WT) and Rbm25-deficiency BMDMs. Methods: Mouse BMDMs were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse M-CSF (20ng/ml). BMDMs were stained to confirm the surface expression of CD11b and F4/80. Cells with purity >97.5% were used for subsequent experiments. WT and Rbm25 deficient BMDMs were stimulated with LPS (100ng/ml) for 4 hours. Results: We carried out CUT&Tag assays using antibody against H3K9ac or H3K27ac, and found the global influence of Rbm25 in regulating H3K9ac and H3K27ac of transcription regulatory regions of key genes for inflammatory macrophage effector function.

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