Project description:To elucidate the potential role of commensal viruses in the etiology of AR, we performed a metagenomic analysis of nasal lavage fluid (NLF) to identify commensal viruses in the nose of mice colonizing in the specific pathogen-free (SPF) circumstances (Vehicle group). To evaluate the potential role of these commensal viruses, we reduced them in the nose of mice by administrating intranasal drops of broad-spectrum antiviral drug ribavirin daily, starting at 4-week-old and lasting for three weeks (Ribavirin group).
Project description:This series includes a 32-array training dataset used to evaluate E-Predict normalization and similarity metric parameters as well as 13 microarrays used as examples in (Urisman, et. al 2005). Training data set includes 15 independent HeLa RNAhybridizations (microarrays 1-15), 10 independent nasal lavage samples positive for Respiratory Syncytial virus (microarrays 16-25), and 7 independent nasal lavage samples positive for Influenza A virus (microarrays 26-32). Examples iclude a serum sample positive for Hepatitis B virus (microarray 33), a nasal lavage sample positive for both Influenza A virus and Respiratory Syncytial virus (microarray 34), and culture samples of 11 distinct Human Rhinovirus serotypes (microarrays 35-45). Keywords = virus detection, E-Predict, species identification, metagenomics
Project description:This series includes a 32-array training dataset used to evaluate E-Predict normalization and similarity metric parameters as well as 13 microarrays used as examples in (Urisman, et. al 2005). Training data set includes 15 independent HeLa RNAhybridizations (microarrays 1-15), 10 independent nasal lavage samples positive for Respiratory Syncytial virus (microarrays 16-25), and 7 independent nasal lavage samples positive for Influenza A virus (microarrays 26-32). Examples iclude a serum sample positive for Hepatitis B virus (microarray 33), a nasal lavage sample positive for both Influenza A virus and Respiratory Syncytial virus (microarray 34), and culture samples of 11 distinct Human Rhinovirus serotypes (microarrays 35-45). Keywords = virus detection, E-Predict, species identification, metagenomics Keywords: other
Project description:Small RNA sequencing was conducted to identify the miRNAs responsible for AR using exosomes isolated from the nasal lavage (NAL) fluid of the control (n=9) and AR patient groups (n=8).
Project description:Induced sputum cells (ISCs) and nasal lavage fluid cells (NLFCs) from 6 patients with CRSwNP, 6 patients suffering from CRSwNP with asthma, and 6 control subjects were procured in the study.
Project description:The study aimed to identify proteins associated with chronic rhinosinusitis with nasal polyps. Samples from nasal Lavage fluid from CRSwNP patients and controls were analysed and revealed a significant difference in protein expression. Dysregulated proteins were linked to airway inflammation, immune response and oxidative stress.
Project description:To explore the impact of nasal commensal viruses on the onset and progression of allergic rhinitis, We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of CD45+ cells in the nasal mucosa of mice treated with Vehicle, Ribavirin, Vehicle-OVA, or Ribavirin-OVA.
Project description:Global patterns of gene expression was profiled in nasal lavage samples obtained from asthmatic children during an acute Picornavirus-induced exacerbation and 7-14 days later. Gene coexpression network analysis and prior knowledge was employed to reconstruct the wiring diagram of the underlying gene networks.
