Project description:Cellular binary fate decisions require the progeny to silence genes associated with the alternative fate. The major subsets of alpha:beta T cells have been extensively studied as a model system for fate decisions. While the transcription factor RUNX3 is required for the initiation of Cd4 silencing in CD8 T cell progenitors, it is not required to maintain the silencing of Cd4 and other helper T lineage genes. The other runt domain containing protein, RUNX1, silences Cd4 in an earlier T cell progenitor, but this silencing is reversed whereas the gene silencing after RUNX3 expression is not reverse. Therefore, we hypothesized that RUNX3 and not RUNX1 recruits other factors that maintains the silencing of helper T lineage genes in CD8 T cells. To this end, we performed a proteomics screen of RUNX1 and RUNX3 to determine candidate silencing factors.
Project description:This SuperSeries is composed of the following subset Series:; GSE7280: Gene expression in peripheral cells: effects of Foxp3 and PDE3B; GSE7770: Gene expression in thymic CD4 T cells: effects of Foxp3 Experiment Overall Design: Thymocyte RNA (GSE7770) was processed with Affy and Enzo reagents whereas peripheral cell RNA (GSE7280) was processed with Ambion reagents, which amplified the cRNA more robustly. As a result, the two data sets don't normalize very well to each other and shouldn't be compared directly to each other.
Project description:We analyzed the individual transcriptomes of thymic Treg cells (CD4+Foxp3+), their immediate precursors (CD25+CD4+Foxp3-) and mature CD4 single positive thymocytes (CD4+Foxp3-CD25-CD62L+CD24-).
Project description:We analyzed open chromatin regions in thymic Treg cells, their immediate precursors (CD25+ Foxp3-), and mature and semi-mature CD4 single positive cells. We wished to understand which chromatin regions become accessible in response to TCR-stimulation and then Foxp3 expression as Treg cells develop.
Project description:Gene expression profiling of CD4+CD25+Foxp3+ regulatory T cells and CD4+CD25-Foxp3- T cells in the course of Plasmodium yoelii infection of BALB/c mice
