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Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei

ABSTRACT: Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei

PROVIDER: PRJNA1055044 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27308480_1.fastq.gz	Fastqsanger.gz
	SRR27308480_2.fastq.gz	Fastqsanger.gz
	SRR27308481_1.fastq.gz	Fastqsanger.gz
	SRR27308481_2.fastq.gz	Fastqsanger.gz
	SRR27308482_1.fastq.gz	Fastqsanger.gz

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Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei [scRNA-seq]

Project description:Brain organoids with nucleus-specific identities provide unique platforms for studying human brain development and diseases at a finer resolution. Despite its essential role in vital body functions, the medulla of the hindbrain has seen a lack of in vitro models, let alone models resembling specific medullary nuclei, including the crucial spinal trigeminal nucleus (SpV) that relays peripheral sensory signals to the thalamus. Here, we report a method to differentiate human pluripotent stem cells into region-specific brain organoids resembling the dorsal domain of the medullary hindbrain. Importantly, organoids specifically recapitulated the development of SpV derived from the dorsal medulla. We also developed an organoid system to create the trigeminothalamic projections between the SpV and the thalamus by fusing these organoids, namely human medullary SpV-like organoids (hmSpVOs), with organoids representing the thalamus (hThOs). Our study provides a platform for understanding SpV development, nucleus-based circuit organizations, and related disorders in the human brain.

2024-08-28 | GSE251679 | GEO

Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei [RNA-seq]

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Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei [RNA-seq]

Project description:Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei [RNA-seq]

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Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei [scRNA-seq]

Project description:Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei [scRNA-seq]

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Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-08-28 | GSE251680 | GEO

Transcriptomic Analysis of Trigeminal Ganglia and Spinal Trigeminal Nucleus Caudalis in Mice with Chronic Inflammatory Temporomandibular Joint Pain

Project description:Purpose: In this study, we aimed to analyze lncRNA expression in the whole transcriptome of trigeminal ganglia (TG) and spinal trigeminal nucleus caudalis (Sp5C) in a chronic inflammatory TMJ pain mouse model. Chronic inflammatory TMJ pain was induced by intra-TMJ injection of complete Freund's adjuvant (CFA). The lncRNA expression patterns in the whole transcriptome of TG and Sp5C were profiled with RNA sequencing.

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Transcriptomic profiling of trigeminal nucleus caudalis (TNC) and spinal cord dorsal horn (SC)

Project description:RNA-Sequencing of the trigeminal nucleus caudalis and spinal cord, dorsal horn in male naive rats (Wistar Han) of 10 weeks old

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DREAM in pain mechanisms in the trigeminal ganglia

Project description:Expression of DREAM in dorsal root ganglia and spinal cord is related to endogenous control mechanisms of acute and chronic pain. In primary sensory trigeminal neurons high levels of endogenous DREAM protein are preferentially localized in the nucleus, suggesting a major transcriptional role. Here, we show that DREAM participates in the control of trigeminal pain perception through the regulation of prodynorphin and BDNF. Furthermore, genome-wide analysis of trigeminal neurons in daDREAM transgenic mice revealed that cathepsin L (CTSL) and the monoglyceride lipase (MGLL) are new DREAM downstream targets and have a role in the regulation of trigeminal nociception.

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Transcriptomic Analysis of Trigeminal Ganglia and Spinal Trigeminal Nucleus Caudalis in Mice with Chronic Inflammatory Temporomandibular Joint Pain

Project description:Transcriptomic Analysis of Trigeminal Ganglia and Spinal Trigeminal Nucleus Caudalis in Mice with Chronic Inflammatory Temporomandibular Joint Pain

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Astroglia in medullary dorsal horn (trigeminal spinal subnucleus caudalis) are involved in trigeminal neuropathic pain mechanisms.

Project description:The aim of this study was to investigate whether astroglia in the medullary dorsal horn (trigeminal spinal subnucleus caudalis; Vc) may be involved in orofacial neuropathic pain following trigeminal nerve injury. The effects of intrathecal administration of the astroglial aconitase inhibitor sodium fluoroacetate (FA) were tested on Vc astroglial hyperactivity [as revealed by glial fibrillary acid protein (GFAP) labeling], nocifensive behavior, Vc extracellular signal-regulated kinase phosphorylation (pERK), and Vc neuronal activity in inferior alveolar nerve-transected (IANX) rats. Compared with sham-control rats, a significant increase occurred in GFAP-positive cells in ipsilateral Vc at postoperative day 7 in IANX rats, which was prevented following FA administration. FA significantly increased the reduced head withdrawal latency to high-intensity heat stimulation of the maxillary whisker pad skin in IANX rats, although it did not significantly affect the reduced escape threshold to low-intensity mechanical stimulation of the whisker skin in IANX rats. FA also significantly reduced the increased number of pERK-like immunoreactive cells in Vc and the enhanced Vc nociceptive neuronal responses following high-intensity skin stimulation that were documented in IANX rats, and glutamine administration restored the enhanced responses. These various findings provide the first documentation that astroglia is involved in the enhanced nociceptive responses of functionally identified Vc nociceptive neurons and in the associated orofacial hyperalgesia following trigeminal nerve injury.

| S-EPMC2804401 | biostudies-literature

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