Project description:Experiment 1<br> Gene profiling upon biotic stress treatment<br> Biological question : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment?<br> Experiment description : 14-day old seedlings (Col-0 and fls2 KO) treated with 10uM of flg22 (flagellin- derived peptide) over a one hour timecourse.<br> <br> Experiment 2<br> Gene profiling in microRNA mutants<br> Biological question : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants?<br> Experiment description : Ler, dcl1-9, hen1-1 and hasty were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after.<br> <br> Experiment 3<br> Gene profiling in siRNA mutants<br> Biological question : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants?<br> Experiment description : Col-0, dcl2-1, dcl3-1 and rdr2-1 were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after.<br> <br> Experiment 4<br> Gene profiling in Agrobacterium-induced tumors and auxin-induced calli.<br> Biological question : What are the genes (including miRNA precursors) that are differentially regulated in an Agrobacterium-induced tumors compared to auxin-induced calli?<br> Experiment description : Col-0 plants were infected with wt Agrobacterium tumefaciens (strain A208) by stabbing the emerging stems. 18 days later, growing tumors were harvested as were the non-infected part of the stabbed-stem.In parallel, Col-0 seeds were germinated on Callus Induction Media (CIM) and 18 days later, calli were harvested.<br> <br> Experiment 5<br> Gene profiling in tasiRNA mutants<br> Biological question : What are the genes (including miRNA precursors) that are differentially regulated in a set of tasiRNA mutants?<br> Experiment description : Col-0, dcl4-1, rdr6-1 were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after.<br> <br> Experiment 6<br> Gene profiling upon biotic stress treatment<br> Biological question : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment?<br> Experiment description : 14-day old seedlings (Col-0 and fls2 KO) treated with 10uM of flg22 (flagellin- derived peptide) over a one hour timecourse.<br> <br> <br>
Project description:rs06-06_mirna - agrobacterium treatment - Time course : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? miRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants? siRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants? - Col-0 plants were infected with wt Agrobacterium tumefaciens (strain A208) by stabbing the emerging stems. 18 days later, growing tumors were harvested as were the non-infected part of the stabbed-stem.In parallel, Col-0 seeds were germinated on Callus Induction Media (CIM) and 18 days later, calli were harvested. Keywords: normal vs disease comparison,organ comparison
Project description:rs06-06_mirna - agrobacterium treatment - Time course : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? miRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants? siRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants? - Col-0 plants were infected with wt Agrobacterium tumefaciens (strain A208) by stabbing the emerging stems. 18 days later, growing tumors were harvested as were the non-infected part of the stabbed-stem.In parallel, Col-0 seeds were germinated on Callus Induction Media (CIM) and 18 days later, calli were harvested. Keywords: normal vs disease comparison,organ comparison 2 dye-swap - CATMA arrays