ABSTRACT: Function verification of a chlorophyll a/b binding protein gene in Kandelia obovata through a newly established tobacco rattle virus-induced gene silencing system
Project description:Function verification of a chlorophyll a/b binding protein gene in Kandelia obovata through a newly established tobacco rattle virus-induced gene silencing system
Project description:Mangrove Kandelia obovata, an important coastal shelterbelt and landscape tree, is distributed in tropical and subtropical shores and likely delimited in the latitudinal range by varying sensitivity to cold. Here, we explored the temporal variations in physiological status and transcriptome profiling of K. obovata under natural frost conditions at ~32oN, as well as the positive role of exogenous abscisic acid (ABA) in cold resistance.
Project description:Blue light (BL) is a fundamental cue for stomatal opening in both C3 and C4 plants. However, it is unknown whether crassulacean acid metabolism (CAM) plants open their stomata in response to BL. We investigated stomatal BL responses in the obligate CAM plants Kalanchoe pinnata and Kalanchoe daigremontiana that characteristically open their stomata at night and close them for part of the day, as contrasted with C3 and C4 plants. Stomata opened in response to weak BL superimposed on background red light in both intact leaves and detached epidermal peels of K. pinnata and K. daigremontiana. BL-dependent stomatal opening was completely inhibited by tautomycin and vanadate, which repress type 1 protein phosphatase and plasma membrane H+-ATPase, respectively. The plasma membrane H+-ATPase activator fusicoccin induced stomatal opening in the dark. Both BL and fusicoccin induced phosphorylation of the guard cell plasma membrane H+-ATPase in K. pinnata. These results indicate that BL-dependent stomatal opening occurs in the obligate CAM plants K. pinnata and K. daigremontiana independently of photosynthetic CO2 assimilation mode.
