Project description:hMSCs were cultured for eight days within perfused three-dimensional matrices under conditions of oscillatory, continuous or static fluid flow of osteogenic media.
2009-09-16 | GSE18114 | GEO
Project description:Microbial diversity in a continuous-flow N-(E)PDA system
| PRJNA1183625 | ENA
Project description:Sulfidogenic continuous-flow systems treated with the specific inhibitor, perchlorate
Project description:In this study, we used an original system to produce C. difficile anaerobic biofilms consisting in continuous-flow microfermentors, that is compared to planktonic growth culture.
Project description:Modulation of mucus production by the human ecto- and endo-cervical epithelium by steroid hormones and associated interactions with commensal microbiome play a central role in the physiology and pathophysiology of the female reproductive tract. Here, we show that in a human organ-on-a-chip model of cervix (Cervix Chip), use of continuous fluid flow promotes ecto-cervical differentiation, whereas use of periodic flow including periods of stasis stimulated endo-cervical specialization. Continuous flow exhibited significant upregulation of genes associated with ectocervical epithelial phenotype whereas the same cells cultured in the same medium under periodic flow or under static conditions in Transwell inserts upregulated genes more closely associated with the endocervical epithelial phenotype. Similar results with minor differences were obtained using epithelial cells isolated from three donors each from a different ethnic background (African American, Hispanic, and Caucasian).
