Paramisgurnus dabryanus
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ABSTRACT:
PROVIDER: PRJNA1057486 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The epidermal bacterial communities structure of Paramisgurnus dabryanus.
| PRJNA504744 | ENA
Project description:The epidermal bacterial communities structure of Paramisgurnus dabryanus.
| PRJNA504745 | ENA
Project description:Paramisgurnus dabryanus Transcriptome sequencing
| PRJNA266739 | ENA
Project description:Paramisgurnus dabryanus Genome sequencing and assembly
| PRJNA911026 | ENA
Project description:Paramisgurnus dabryanus Raw sequence reads
| PRJNA562989 | ENA
Project description:Most currently available bioreactors have some defects in the expression, activity, or purification of target protein and peptide molecules, whereas the mucus gland of fish can overcome these defects to become a novel bioreactor for the biopharmaceutical industry. In this study, we have evaluated the practicability of developing a mucus gland bioreactor in loach (Paramisgurnus dabryanus). A transgenic construct pT2-krt8-IFN1 was obtained by subcloning the promoter of zebrafish keratin 8 gene and the type I interferon (IFN1) cDNA of grass carp into the SB transposon. The IFN1 expressed in CIK cells exhibited an antiviral activity against the replication of GCRV873 and activated two genes downstream of JAK-STAT signaling pathway. A transgenic loach line was then generated by microinjection of the pT2-krt8-IFN1 plasmids and in vitro synthesized capped SB11 mRNA. Southern blots indicated that a single copy of IFN1 gene was stably integrated into the genome of transgenic loach. The expression of grass carp IFN1 in transgenic loaches was detected with RT-PCR and Western blots. About 0.0825 µg of grass carp IFN1 was detected in 20 µL mucus from transgenic loaches. At a viral titer of 1 × 103 PFU/mL, plaque numbers on plates containing mucus from transgenic loaches reduced by 18% in comparison with those of the control, indicating that mucus of IFN1-transgenic loaches exhibited an antiviral activity. Thus, we have successfully created a mucus gland bioreactor that has great potential for the production of various proteins and peptides.
| S-EPMC7827776 | biostudies-literature
Project description:Taiwanese loach was bred in Formosa and then widely cultivated in China. Because of overfishing and environmental pollution, the number of wild Taiwanese loach has been sharply decreased in these years. Also the taxonomic status of Taiwanese loach is still unclear. In this study, the complete mitochondrial genome of Taiwanese loach was obtained by PCR. The genome is 16,569 bp in length, including 2 ribosomal RNA genes, 13 proteins-coding genes, 22 transfer RNA genes, and a non-coding control region, the gene composition and order of which was similar to most reported from other vertebrates. Sequence analysis showed that the overall base composition is 29.5% for A, 27.5% for T, 26.4% for C, and 16.6% for G. The sequence is a slight A + T bias of 57.0%. By analyzing phylogenetic analysis and BLAST, the similarity to Paramisgurnus was >99%, we dare to speculate that the Taiwanese loach cultivated in China was a subspecies of P. dabryanus. Mitogenome information from this study could be a useful basis for conservation and phylogenetics of Taiwanese loach, P. dabryanus ssp.
| S-EPMC7800862 | biostudies-literature
Project description:Paramisgurnus dabryanus isolate:BB Genome sequencing and assembly
| PRJNA980909 | ENA
Project description:The hybrid loach of P. dabryanus ssp. (female) and M. anguillicaudatus (male) has the desirable trait of growth performance. There is no report of the complete genome of this hybrid. In this study, the complete mitochondrial genome of this hybrid loach was obtained, and the genome is 16,569 bp in length, including two ribosomal RNA genes. Thirteen proteins-coding genes, 22 transfer RNA genes, and a non-coding control region, the gene composition and order of which was similar to most reported from other vertebrates. Sequence analysis showed that the overall base composition is 29.5% for A, 27.5% for T, 26.4% for C, and 16.6% for G. The sequence is a slight A + T bias of 57.0%. The phylogenetic tree showed that the hybrid loach to be one of Paramisgurnus, and the relationships of M. anguillicaudatus were closer. Also the mitochondrial genome sequence of loach were aligned by BLAST, compared with Cobitinae the sequence similarity could reach >90%, and the similarity to Paramisgurnus was >99%. Mitogenome information from this study could be a useful basis for conservation and phylogenetics of this hybrid loach.
| S-EPMC7800976 | biostudies-literature