Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Direct Transposition of Native DNA for Sensitive Multimodal Single-molecule Sequencing

ABSTRACT: Direct Transposition of Native DNA for Sensitive Multimodal Single-molecule Sequencing

PROVIDER: PRJNA1058794 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Direct transposition of native DNA for sensitive multimodal single-molecule sequencing

Project description:Direct transposition of native DNA for sensitive multimodal single-molecule sequencing

| PRJNA934945 | ENA

Direct Transposition of Native DNA for Sensitive Multimodal Single-molecule Sequencing

Project description:Direct Transposition of Native DNA for Sensitive Multimodal Single-molecule Sequencing

| PRJNA1058793 | ENA

Direct transposition of native DNA for sensitive multimodal single-molecule sequencing I

Project description:Direct transposition of native DNA for sensitive multimodal single-molecule sequencing I

| PRJNA864052 | ENA

Direct transposition of native DNA for sensitive multimodal single-molecule sequencing IV

Project description:Direct transposition of native DNA for sensitive multimodal single-molecule sequencing IV

| PRJNA1030419 | ENA

Direct transposition of native DNA for sensitive multimodal single-molecule sequencing III

Project description:Direct transposition of native DNA for sensitive multimodal single-molecule sequencing III

| PRJNA934948 | ENA

Direct transposition of native DNA for sensitive multimodal single-molecule sequencing II

Project description:Direct transposition of native DNA for sensitive multimodal single-molecule sequencing II

| PRJNA934947 | ENA

Direct transposition of native DNA for sensitive multimodal single-molecule sequencing IV

Project description:Concurrent readout of sequence and base modifications from long unamplified DNA templates by PacBio single-molecule sequencing requires large amounts of input material. Here we adapt Tn5 transposition to introduce hairpin oligonucleotides and fragment (tagment) limiting quantities of DNA for generating PacBio-compatible circular molecules. We developed two methods that implement tagmentation and use 90–99% less input than current protocols: (1) single-molecule real-time sequencing by tagmentation (SMRT-Tag), which allows detection of genetic variation and CpG methylation; and (2) single-molecule adenine-methylated oligonucleosome sequencing assay by tagmentation (SAMOSA-Tag), which uses exogenous adenine methylation to add a third channel for probing chromatin accessibility. SMRT-Tag of 40 ng or more human DNA (approximately 7,000 cell equivalents) yielded data comparable to gold standard whole-genome and bisulfite sequencing. SAMOSA-Tag of 30,000–50,000 nuclei resolved single-fiber chromatin structure, CTCF binding and DNA methylation in patient-derived prostate cancer xenografts and uncovered metastasis-associated global epigenome disorganization. Tagmentation thus promises to enable sensitive, scalable and multimodal single-molecule genomics for diverse basic and clinical applications.

2024-05-01 | GSE245946 | GEO

Direct transposition of native DNA for sensitive multimodal single-molecule sequencing II

2024-05-01 | GSE225310 | GEO

Direct transposition of native DNA for sensitive multimodal single-molecule sequencing I

2022-08-08 | GSE210136 | GEO

Direct transposition of native DNA for sensitive multimodal single-molecule sequencing III

2024-05-01 | GSE225311 | GEO