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ABSTRACT: ITS sequencing of the pine trees related soil in Panax notginseng-pine forest agroforestry system

PROVIDER: PRJNA1058851 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27377632_1.fastq.gz	Fastqsanger.gz
	SRR27377632_2.fastq.gz	Fastqsanger.gz
	SRR27377633_1.fastq.gz	Fastqsanger.gz
	SRR27377633_2.fastq.gz	Fastqsanger.gz
	SRR27377634_1.fastq.gz	Fastqsanger.gz

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Project description:16SRNA sequencing of the Panax notginseng-pine forest agroforestry system

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Soil exposure modulates the immune response to an influenza challenge in a mouse model

Project description:This study began with 72 male 4-week-old BALB/c mice. The mice were split evenly into one of four cohorts: Control, River, Pine, and Road. The control mice were raised with standard corn cob bedding whereas the remaining mice were raised with clean bedding amended with 300 mL of one of three different types of soil. The soil exposure continued throughout the experiment, with 300 mL of new soil added with bi-weekly cage changes. The soils used to amend the cage bedding were previously characterized as having high (Pine), medium (River), and low (Road) diversity. The River and Pine soil were collected from Duke Forest and the Road soil was collected adjacent to Highway 15-501 in Chapel Hill, North Carolina. All mice were given a standard diet and the cages were distributed reverse osmosis treated water through a centralized Lixit® system that was fed to each cage in parallel. After 32 days of standard rearing with amended soils, the mice were exposed via oropharyngeal aspiration to either live influenza A (PR8) virus or heat inactivated (HI) virus.

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