Project description:Transcriptomes of thyroid hormone-exposed Rana [Lithobates] catesbeiana cultured back skin in warm, cold, and temperature shift conditions
Project description:Transcriptomes of thyroid hormone-exposed Rana [Lithobates] catesbeiana tadpole tissues at metamorphically permissive, nonpermissive, or a shift from nonpermissive to permissive temperatures
Project description:Effects of Acute Exposure to the Non-steroidal Anti-inflammatory Drug Ibuprofen on the Developing North American Bullfrog (Rana catesbeiana) Tadpole
Project description:Anuran metamorphosis is characterized by profound morphological changes including remodeling of tissues and organs. Here we investigate serum of Rana [Lithobates] catesbeiana (n=5-10) across seven distinct postembryonic stages beginning with premetamorphic tadpole (Gosner stage 31-33) and continuing through metamorphosis to a juvenile frog (Gosner stage 46). Using a sensitive nanoLC-Orbitrap system an untargeted analysis workflow was applied. Among 6,062 detected endogenous metabolites, 421 showed significant metamorphosis-dependent concentration dynamics. Among these potential bioindicators were several prostaglandins and other eicosanoids, some steroid hormones including testosterone, and several carnitines. These data provide a necessary context for interpreting developmental processes and potential molecular crosstalk mechanisms. Furthermore, this provides a solid foundation from which alternative bioindicators of metamorphosis may be chosen as feasible endpoints in future risk assessment of chemicals and their potency for causing developmental toxicity.
