Project description:The diversity and environmental distribution of the nosZ gene, which encodes the enzyme responsible for the consumption of nitrous oxide, was investigated in marine and terrestrial environments using a functional gene microarray. The microbial communities represented by the nosZ gene probes showed strong biogeographical separation, with communities from surface ocean waters and agricultural soils significantly different from each other and from those in oceanic oxygen minimum zones. Atypical nosZ genes, usually associated with incomplete denitrification pathways, were detected in all the environments, including surface ocean waters. The abundance of nosZ genes, as estimated by quantitative PCR, was highest in the agricultural soils and lowest in surface ocean waters.
Project description:The aim was to study the physiological functions of Class II TPS proteins in Tre6P and sugar signaling. For that - T-DNA insertion knockout mutants were identified for all class II TPS genes, then a combinatorial mutants were generated: and tps567 (clade I) knockout and tps891011 (clade II) knockouts were compared to WT.
Project description:Shotgun proteomics focusing on the membrane proteomes, of four Synechococcus spp. strains namely CC9311 (clade I), CC9605 (Clade II), WH8102 (clade III) and CC9902 (clade IV) were conducted to gain insight into the amount of resources these unicellular organisms invest into adaptation strategies.
