Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1061215 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR27429275_1.fastq.gz | Fastqsanger.gz | |||
| SRR27429275_2.fastq.gz | Fastqsanger.gz | |||
| SRR27429276_1.fastq.gz | Fastqsanger.gz | |||
| SRR27429276_2.fastq.gz | Fastqsanger.gz | |||
| SRR27429277_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 36 |
Similar Datasets
Project description:Epigenetic modifications play essential roles in regulating gene expression, yet establishing direct causality between specific modifications and transcriptional outcomes remains challenging. In this study, we employed a CRISPR/dCas9-SunTag system to specifically reduce H3K27me3 levels at the FLOWERING LOCUS T (FT) gene in Arabidopsis thaliana. Targeting a truncated version of the H3K27 demethylase REF6 lacking its DNA-binding zinc fingers (REF6ΔZnF) to the FT locus effectively decreased local H3K27me3, enhanced FT transcription, and led to early flowering phenotypes, particularly under non-inductive short-day (SD) conditions. Chromatin immunoprecipitation confirmed the site-specific reduction of H3K27me3, with minimal genome-wide changes. Under long-day (LD) conditions, H3K27me3 levels at FT oscillated diurnally, coinciding with FT induction at dusk, while in SD conditions, H3K27me3 remained stable, contributing to FT repression. Our results provide direct evidence that H3K27me3 represses FT transcription under non-inductive conditions and demonstrate the utility of epigenetic editing for fine-tuning gene expression and developmental traits in plants
2025-11-20 | GSE303376 | GEO
Project description:The atypical cadherin fat (ft) controls growth, planar cell polarity (PCP) tissue organization, and mitochondrial function, in organisms ranging from fruit flies to mammals. Working at the apical-junctional plasma membrane, the intracellular domain of the Ft protein, FtICD, binds to and regulates components of the Hippo and PCP pathways. Unexpectedly, we find that a fragment of the FtICD is present in the nucleus in cultured cells as well as in embryonic and larval tissues, and we identify nuclear localization and nuclear export signals in FtICD required for this localization. We show that membrane-bound FtICD is cleaved and enters nuclei in vivo. Using endogenously tagged Ft as well as overexpressed FtICD, we conducted ChIP-seq experiments and identified putative Ft targets, including genes involved in signaling pathways, chromatin organization, pattern formation, and neural development. RNAseq demonstrates that some of these genes are dysregulated in ft mutants. We observe strong co-localization of Ft binding regions with peaks for other factors such as DREF and BEAF-32, as well as the Hippo pathway components Yorkie (Yki) and Scalloped (Sd), suggesting that Ft may act in conjunction with these factors to regulate gene expression. Supporting this hypothesis, we found that Ft physiclly interacts with both Yki and Sd in co-immunoprecipitation experients in S2 cells. We propose that the modulation of Hippo pathway activity constitutes one of the nuclear functions of Ft, complementing its estalished function as an upstream regulator of Hippo signaling.
2025-09-25 | GSE308417 | GEO
Project description:To identify key genes in the regulation of salt tolerance in the mangrove plant Bruguiera gymnorhiza, the transcriptome profiling under salt stress was carried out. Main roots and lateral roots were collected from the mangrove plants at 0, 1, 3, 6, 12 and 24 h, 3 6 and 12 days after NaCl-treatment. Keywords: time course, stress response, root type comparison
2008-05-01 | GSE10942 | GEO
Project description:Plants align flowering with optimal seasonal conditions to increase reproductive success. This process depends on modulating signalling pathways that respond to diverse environmental and hormonal inputs, thereby regulating the transition to flowering at the shoot apical meristem. In Arabidopsis, long-day photoperiods (LDs) stimulate the transcription of FLOWERING LOCUS T (FT), encoding the main florigenic signal. FT activation is mediated by the transcriptional regulator CONSTANS (CO), which binds to the CO responsive elements (COREs) located in the proximal FT promoter region. The phytohormone abscisic acid also (ABA) contributes to FT activation together with GIGANTEA (GI) to regulate drought escape (DE). Whether CO is a target of ABA and GI actions for the regulation of FT is, however, unknown. Here we report that ABA and its signalling components promote CO recruitment to the COREs, without causing clear effects on the diel pattern of CO protein accumulation. We also found that GI promotes CO recruitment to the COREs region, and that CO recruitment is required for the accumulation of RNAPol II at the TRANSCRIPTION START SITE of FT. Finally, we show that GI and ABA signalling pathways are largely epistatic in the control of flowering time, suggesting their involvement in the same molecular process. Taken together, these observations suggest that varying water deficit conditions modulate CO recruitment and FT expression, thus dictating DE strategies in Arabidopsis.
2025-05-13 | GSE291256 | GEO
Project description:high throughput sequencing of the metagenome of mexican children
2020-03-16 | GSE143207 | GEO
Project description:Mouse skin 16S amplicon from ft/ft and ft/ftMyD88-/- mice
| PRJNA1276238 | ENA
Project description:Flowering of Arabidopsis thaliana is accelerated by several environmental cues, including exposure to long days. The photoperiod-dependent promotion of flowering involves the transcriptional induction of FLOWERING LOCUS T (FT) in the phloem of the leaf. FT encodes a mobile protein that is transported from the leaves to the shoot apical meristem, where it forms part of a regulatory complex that induces flowering. Whether FT also has biological functions in leaves of wild-type plants remains unclear. In order to address this issue, we first studied the leaf transcriptomic changes associated with its over expression in the companion cells of the phloem. To this end, transgenic A. thaliana plants that misexpress FT from the pGAS1 promoter in a ft-10 tsf-1 double mutant background were employed (pGAS1:FT ft-10 tsf-1). In these transgenic plants, the use of the pGAS1 promoter ensures that the FT transgene is expressed in phloem companion cells of the minor veins, recreating the spatial pattern of expression described for the native gene. In this studuy, the transcriptome of leaves of pGAS1:FT ft-10 tsf-1 transgenic plants was compared to that of ft-10 tsf-1 and Col-0 plants using Tiling Arrays.
2020-02-05 | GSE125054 | GEO
Project description:The precise onset of flowering is crucial to ensure successful plant reproduction. The gene FLOWERING LOCUS T (FT) encodes florigen, a mobile signal that is produced in leaves and initiates flowering in the shoot apical meristem. FT is highly expressed in phloem companion cells that reside in distal leaf regions. Thus far, a detailed molecular characterization of the FT-expressing phloem companion cells has been lacking. Here, we used single-nuclei RNA-seq to investigate gene expression in FT-expressing cells and other phloem companion cells. We discovered that companion cells with high FT expression form a unique cluster and express other genes encoding small proteins, including the anti-florigen BROTHER OF FT (BFT). The promoters of FT and the genes co-expressed with FT were enriched for the consensus binding motif of NITRATE-INDUCIBLE GARP-TYPE TRANSCRIPTIONAL REPRESSOR1 (NIGT1). Overexpression of the paralogs NIGT1.2 and NIGT1.4 in transgenic Arabidopsis lines significantly delayed flowering under nitrogen-rich conditions, consistent with NIGT1s acting as nitrogen-dependent FT repressors. Taken together, our results demonstrate that FT-expressing cells show a distinct expression profile that suggests that these cells produce multiple systemic signals to regulate plant growth and development.
2025-10-03 | GSE273032 | GEO