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A developmentally-inspired hypoxia condition promotes kidney organoids differentiation from human pluripotent stem cells

ABSTRACT: A developmentally-inspired hypoxia condition promotes kidney organoids differentiation from human pluripotent stem cells

PROVIDER: PRJNA1061548 | ENA |

REPOSITORIES: ENA

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A developmentally-inspired hypoxia condition promotes kidney organoids differentiation from human pluripotent stem cells

Project description:Kidney organoids derived from human pluripotent stem cells (hPSCs) lack a contiguous network of collecting ducts, which limits their utility in modeling kidney development and disease. Here, we report the generation of kidney organoids containing ureteric bud (UB)-derived collecting ducts connected to metanephric mesenchyme (MM)-derived nephrons using a developmentally-inspired hypoxic differentiation condition. Hypoxia promotes a reiterative process of branching morphogenesis and nephron induction through reciprocal interactions between co-induced MM and UB, which lead to a higher-order kidney organogenesis in vitro. The resulting kidney organoids demonstrate greater maturity, as indicated by higher levels of functional markers and a more realistic micro-anatomy. Additionally, these hypoxic-enhanced kidney organoids show a great potential as in vitro models for renal cystic diseases, as they efficiently generate cystic formations and display high sensitivity to drugs. This hypoxia approach may open new avenues for an enhanced understanding of kidney development and diseases.

2025-01-05 | GSE252639 | GEO

Regulation of chromatin accessibility in hypoxia condition

Project description:Regulation of chromatin accessibility in hypoxia condition

| PRJNA826504 | ENA

Different LncRNAs in exosomes derived from skov-3 cells when exposed to normal or hypoxia condition

Project description:reports shows that EOC cell under hypoxia condition shows high malignancy. So we use the Arraystar Human LncRNA Microarray V3.0 to verify the difference expression of LncRNA in the exosomes drived from skov-3 cells which cultured in nomor/hypoxia condition

2019-12-16 | GSE92498 | GEO

Genome-wide maps of HIF1a binding sites in ccRCC cell line under hypoxia condition

Project description:We report the application of chromatin immunoprecipitation and next generation sequencing technology for HIF1a binding sites at genome wide level in a RCC (renal cell carcinoma) cell line under hypoxia conditions. We found HIF1a binding sites in Caki-2 cell line under hypoxia conditions. Especially, we found HIF1a bind to SPOP under hypoxia condition, which was further validated.

2014-03-27 | GSE54327 | GEO

Genome-wide maps of HIF1a binding sites in ccRCC cell line under hypoxia condition

2014-03-27 | E-GEOD-54327 | biostudies-arrayexpress

Zebrafish developmental hypoxia

Project description:Study of hypoxia-induced differential expression during zebrafish development at gastrulation (shield) and segmentation (8-somite) stages An 8-chip study with 2 biological replicates from +/- hypoxia at 2 different stages of development. Each sample is prepared from 12-15 dechorionated, hand-selected, developmentally synchronized embryos exposed to 1-2 hours of hypoxia.

2015-05-02 | E-GEOD-68473 | biostudies-arrayexpress

Regulation of chromatin accessibility in hypoxia condition

Project description:Hypoxia leads to significant changes at the histone modification marks, including increasing methylation of H3K4, H3K9 and H3K27. However, the overall effect on chromatin accessibility is not fully understood. Here, we employed an ATACseq method on PFA-fixed mouse glioma cell line GL261to test the genomewide chromatin accessibility changes in response to two hypoxic conditions: moderate hypoxia (1% O2) and severe hypoxia (<0.1% O2).

2023-03-05 | GSE200757 | GEO

Transcriptomics Modeling of the Late-Gestation Fetal Pituitary Response to Transient Hypoxia

Project description:The late-gestation fetal sheep responds to hypoxia with physiological, neuroendocrine, and cellular responses that aid in fetal survival . The response of the fetus to hypoxia represents a coordinated effort to maximize oxygen transfer from the mother and minimize wasteful oxygen consumption by the fetus. While there have been many studies aimed at investigating the coordinated physiological and endocrine responses to hypoxia, and while immunohistochemical or in situ hybridization studies have revealed pathways supporting the endocrine function of the pituitary, there is little known about the coordinated cellular response of the pituitary to the hypoxia. The objective of this study was to use transcriptomics and systems analysis to determine significantly altered biological processes in the late gestation ovine fetal pituitary one hour after a 30 minute period of hypoxia, produced by lowering the inspired oxygen content in the maternal inspired gas. We found that the acute response to 30 min of transient hypoxia in the late-gestation fetus results in reduced cellular metabolism and a pattern of gene expression that is consistent with cellular oxygen and ATP starvation. The response is not consistent with gene regulation by HIF1A .

2015-10-01 | GSE69246 | GEO

Extracellular Vesicles from Hypoxia Culture of Human Mesenchymal Stem Cells

Project description:In current study, hMSCs were grown under Hypoxia condition. mRNA sequencing reveals global transcriptome alterations for hypoxia condition.

2023-04-27 | GSE229825 | GEO

lncRNA SCIRT in hypoxia/reoxygenation.

Project description:To know effects of long noncoding RNA (lncRNA) SCIRT in hypoxia/reoxygenation (H/R). We compared expression profiles of human umbilical vein endothelial cells (HUVECs) under normal condition, H/R condition without SCIRT knockdown, and H/R condition with SCIRT knockdown.

2022-06-15 | GSE193048 | GEO

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