Project description:Batch cultures of Wild-type C. jejuni NCTC11168 were grown in 150 ml volumes of Mueller-Hinton broth in 250 ml baffled flasks. Microaerophilic conditions were generated using a MACS-VA500 microaerophilic work station (10 % O2, 10 % CO2, 80 % N2) from Don Whitley Scientific, Ltd which also maintained the growth temperature at 42 ºC. When mid-exponential phase was reached 0.25 mM GSNO was added to half of the cultures. After a 10 minute exposure samples of both treated and untreated cells were harvested into phenol/ethenol to stabilize the RNA and total RNA was purified using Qiagen’s RNeasy Mini kit (as recommended by the suppliers) prior to use in microarray analysis. Keywords: Stress-response of batch cultures to GSNO

2007-12-31 | GSE5397 | GEO

Desmodesmus cultures

Project description:Desmodesmus cultures Raw sequence reads

| PRJNA990817 | ENA

Anaerobic enriched-cultures

Project description:Anaerobic enriched-cultures Raw sequence reads

| PRJNA818155 | ENA

Assessing the effects of transcription factor knockouts on growth in high-density fed-batch cultures

Project description:We used RNA-seq to investigate the effects of single transcription factor knockouts on growth of E. coli BW25113 in fed-batch cultures at multiple timepoints. Cells were grown in an ambr automated bioreactor and maintained at 30 degrees and pH 6.5 in Medium C.

2022-12-30 | GSE221706 | GEO